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D218 Sigma

Monoclonal Anti-Dihydropyridine Receptor (α1 Subunit) antibody produced in mouse

clone 1A, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Calcium Channels, Antibodies to Ion Channels,
species reactivity   guinea pig, human (weakly), rabbit, rat, mouse
application(s)   immunohistochemistry (frozen sections): 1:200
  immunoprecipitation: suitable
  western blot (chemiluminescent): 1:500
clone   1A, monoclonal
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt ~200 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CACNA1S(779)
mouse ... Cacna1s(12292)
rat ... Cacna1s(116652)
conjugate   unconjugated

Description

Immunogen

rabbit muscle transverse tubule dihydropyridine (DHP) receptors.

General description

DHP receptor is composed of at least four subunits. The α1 subunit contains the binding site for the DHPs and shows high sequence homology to the voltage gated Na+ channel. The α2 subunit is a large glycoprotein and the other 2 subunits are termed β and γ.

Physical form

Solution in phosphate buffered saline containing 0.05% sodium azide.

Specificity

Reacts specifically with the α1 subunit of the DHP receptor. May be used to inhibit the DHP-sensitive calcium current in BC3H1 mouse muscle cells. By immunohistochemistry, double rows of discrete punctate staining represent pairs of triads on the opposing sides of the Z-lines.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Monoclonal Anti-Dihydropyridine Receptor (α1 Subunit) antibody produced in mouse is suitable for immunoprecipitation, immunohistochemistry at a working dilution of 1:500 and immunoblotting at 1:2000. It was used for the detection and localization of DHPR-1α by western blotting in skeletal muscle tissue sections of mice in a study. The antibody may be used to inhibit the DHP-sensitive calcium current in BC3H1 mouse muscle cells.

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Biochem/physiol Actions

The 1,4-dihydropyridines (DHPs) are synthetic organic compounds which can be used to identify the L-type Ca2+ channels that are found in all types of vertebrate muscle, neuronal and neuroendocrine cells. DHP receptor is part of the L-type calcium channel complex and is thought to be the voltage sensor in E-C coupling.

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Safety & Documentation

Documents

Certificate of Analysis

Certificate of Origin

Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  nwg
Protocols & Articles

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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