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D2438 Sigma

Dimethyl sulfoxide

sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma

Synonym: DMSO

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Properties

Related Categories Apoptosis and Cell Cycle, Cell Biology, Cell Culture, Cell Freezing Media and Reagents, Cell Signaling and Neuroscience,
Quality Level   GMP
grade   BioPerformance Certified
vapor density   2.7 (vs air)
vapor pressure   0.42 mmHg ( 20 °C)
InChI Key   IAZDPXIOMUYVGZ-UHFFFAOYSA-N
sterility   sterile-filtered
assay   (USP XXIV)
autoignition temp.   573 °F
expl. lim.   42 %, 63 °F
color   colorless
refractive index   n20/D 1.479(lit.)
bp   189 °C(lit.)
mp   16-19 °C(lit.)
solubility   H2O: miscible (completely)
density   1.10 g/mL(lit.)
suitability   meets EP, USP testing specifications
  suitable for hybridoma

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Human and animal cell lines grown in culture are generally stored frozen. Freezing protects the cell line from changes due to genetic drift and minimizes risk of contamination. Liquid nitrogen used in conjunction with a cryoprotective agent such as DMSO is a widely used method for preserving cells. Without the presence of a cryoprotective agent, freezing is lethal to most mammalian cells. Damage is caused by mechanical injury by ice crystals, concentration of electrolytes, pH changes, and denaturation of proteins. These lethal effects are minimized by adding a cryoprotective agent which lowers the freezing point and allows for a slower cooling rate. BPC-tested DMSO also meets the requirements of biotechnology and tissue engineering applications.

Caution

Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.

Features and Benefits

Ready-to-use sterile filtered product conveniently packaged in septum screw-capped amber bottles. Meets USP and EP testing requirements

Price and Availability


Filter out uncertainty.

All labs need water
Safety & Documentation

Safety Information

RIDADR 
NA 1993 / PGIII
WGK Germany 
1
RTECS 
PV6210000
Flash Point(F) 
188.6 °F
Flash Point(C) 
87 °C
Protocols & Articles
Peer-Reviewed Papers
15

References

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