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  • D2630 - 2,4-Dinitrophenylhydrazine


D2630 Sigma


  • CAS Number 119-26-6

  • Linear Formula (O2N)2C6H3NHNH2

  • Molecular Weight 198.14

  •  Beilstein Registry Number 615586

  •  MDL number MFCD00007578

  • Popular Documents:  FTNMR (PDF)  



contains   ≥30% water as stabilizer (to comply with DOT regulations)
mp   197-200 °C(lit.)

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puriss. p.a., moistened with water, ≥99.0% (HPLC)

2,4-<WBR>Dinitrophenylhydrazine Phosphoric acid solution

for derivatization (of aldehydes and ketones), substitute for the explosive solid, ~0.2 M (~4%)

Safety & Documentation

Safety Information

Signal word 
Hazard statements 
Precautionary statements 
UN 3380 4.1/PG 1
WGK Germany 

Protocols & Articles

Peer-Reviewed Papers


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A quick, convenient and economical method for the reliable determination of methylglyoxal in millimolar concentrations: the N-acetyl-L-cysteine assay. Wild R, Ooi L, Srikanth V, et al. Anal. Bioanal. Chem 403(9), 2577-81, (2012)


[Modification of spectrophotometric method for determination of protein carbonyl groups]. Zaĭtseva OV and Shandrenko SH Ukr. Biokhim. Zh. 84(5), 112-6, (2012)


Analysis of volatile aldehyde biomarkers in human blood by derivatization and dispersive liquid-liquid microextraction based on solidification of floating organic droplet method by high performance liquid chromatography. Lili L, Xu H, Song D, et al. J. Chromatogr. A. 1217(16), 2365-70, (2010)


Simultaneous removal of heavy-metal ions in wastewater samples using nano-alumina modified with 2,4-dinitrophenylhydrazine. Afkhami A, Saber-Tehrani M, and Bagheri H J. Hazard. Mater. 181(1-3), 836-44, (2010)


[13C2]-Acetaldehyde promotes unequivocal formation of 1,N2-propano-2'-deoxyguanosine in human cells. Garcia CC, Angeli JP, Freitas FP, et al. J. Am. Chem. Soc. 133(24), 9140-3, (2011)


Determination of acrolein and other carbonyls in cigarette smoke using coupled silica cartridges impregnated with hydroquinone and 2,4-dinitrophenylhydrazine. Uchiyama S, Inaba Y, and Kunugita N J. Chromatogr. A. 1217(26), 4383-8, (2010)


Photocatalytic removal of NO and HCHO over nanocrystalline Zn2SnO4 microcubes for indoor air purification. Ai Z, Lee S, Huang Y, et al. J. Hazard. Mater. 179(1-3), 141-50, (2010)


A novel solid-phase microextraction method based on polymer monolith frit combining with high-performance liquid chromatography for determination of aldehydes in biological samples. Xu H, Wang S, Zhang G, et al. Anal. Chim. Acta 690(1), 86-93, (2011)


Identification of carbonylated peptides by tandem mass spectrometry using a precursor ion-like scan in negative ion mode. Bollineni RCh, Fedorova M, and Hoffmann R J. Proteomics 74(11), 2351-9, (2011)


Capture of the volatile carbonyl metabolite of flecainide on 2,4-dinitrophenylhydrazine cartridge for quantitation by stable-isotope dilution mass spectrometry coupled with chromatography. Prokai L, Szarka S, Wang X, et al. J. Chromatogr. A. 1232, 281-7, (2012)


Proteomic approaches to identifying carbonylated proteins in brain tissue. Linares M, Marín-Garcíía P, Méndez D, et al. J. Proteome Res. 10(4), 1719-27, (2011)


Derivatization of carbonyl compounds with 2,4-dinitrophenylhydrazine and their subsequent determination by high-performance liquid chromatography. Uchiyama S, Inaba Y, and Kunugita N J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci. 879(17-18), 1282-9, (2011)


Distribution of protein oxidation products in the proteome of thermally processed milk. Meyer B, Baum F, Vollmer G, et al. J. Agric. Food Chem. 60(29), 7306-11, (2012)


Evaluation of Alternate Isotope-Coded Derivatization Assay (AIDA) in the LC-MS/MS analysis of aldehydes in exhaled breath condensate. Manini P, Andreoli R, Sforza S, et al. J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci. 878(27), 2616-22, (2010)


Denuder sampling techniques for the determination of gas-phase carbonyl compounds: a comparison and characterisation of in situ and ex situ derivatisation methods. Kahnt A, Iinuma Y, Böge O, et al. J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci. 879(17-18), 1402-11, (2011)


A diffusive sampling device for simultaneous determination of ozone and carbonyls. Uchiyama S, Inaba Y, and Kunugita N Anal. Chim. Acta 691(1-2), 119-24, (2011)


Isolation of enantioselective α-hydroxyacid dehydrogenases based on a high-throughput screening method. Xue YP, Wang W, Wang YJ, et al. Bioprocess Biosyst. Eng. 35(9), 1515-22, (2012)


Formation pathways of gamma-butyrolactone from the furan ring of tegafur during its conversion to 5-fluorouracil. Yamamiya I, Yoshisue K, Matsushima E, et al. Drug Metab. Dispos. 38(8), 1267-76, (2010)


Semi-automated image analysis: detecting carbonylation in subcellular regions of skeletal muscle. Kostal V, Levar K, Swift M, et al. Anal. Bioanal. Chem 400(1), 213-22, (2011)


An optimized method for the measurement of acetaldehyde by high-performance liquid chromatography. Guan X, Rubin E, and Anni H Alcohol. Clin. Exp. Res. 36(3), 398-405, (2012)


A simple and high collection efficiency sampling method for monitoring of carbonyl compounds in a workplace environment. Bunkoed O, Thavarungkul P, Thammakhet C, et al. J. Environ. Sci. Health. A. Tox. Hazard. Subst. Environ. Eng. 47(2), 167-75, (2012)


Underestimation of pyruvic acid concentrations by fructose and cysteine in 2,4-dinitrophenylhydrazine-mediated onion pungency test. Yoo KS, Lee EJ, and Patil BS J. Food Sci. 76(8), C1136-42, (2011)


A spectrophotometric assay method for vanadium in biological and environmental samples using 2,4-dinitrophenylhydrazine with imipramine hydrochloride. Al-Tayar NG, Nagaraja P, Vasantha RA, et al. Environ. Monit. Assess. 184(1), 181-91, (2012)


Evaluation of cost-effective sol-gel-based sensor for monitoring of formaldehyde in workplace environment and cancer risk assessment. Bunkoed O, Thavarungkul P, Thammakhet C, et al. J. Environ. Sci. Health. A. Tox. Hazard. Subst. Environ. Eng. 48(3), 263-72, (2013)


Autoimmune reactivity of IgM acquired after oxidation. Omersel J, Avberšek-Lužnik I, Grabnar PA, et al. Redox Rep. 16(6), 248-56, (2011)


Merck 14,3283

Beil. 15,IV,380

Fieser 1,330 / Fieser 2,176

Aldrich MSDS 1, 816:D / Corp MSDS 1 (1), 1440:D / FT-IR 1 (1), 1371:D / FT-IR 2 (2), 2317:A / FT-NMR 1 (2), 752:C / IR-Spectra (3), 829:C / IR-Spectra (2), 726:H / NMR-Reference 2 (1), 1175:C / RegBook 1 (1), 1589:H / Sax 6, 1217 / Sigma FT-IR 1 (2), 518:A / Structure Index 1, 247:B:8

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D199303 2,4-Dinitrophenylhydrazine, reagent grade, 97%

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