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D2650 Sigma

Dimethyl sulfoxide

Hybri-Max, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%

Synonym: DMSO

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Properties

Related Categories BRDU Cell Proliferation Assay Reagents, Cell Biology, Cell Culture, Cell Freezing Media and Reagents, Cell Signaling and Neuroscience,
Quality Level   PREMIUM
grade   Hybri-Max
vapor density   2.7 (vs air)
vapor pressure   0.42 mmHg ( 20 °C)
InChI Key   IAZDPXIOMUYVGZ-UHFFFAOYSA-N
sterility   sterile-filtered
product line   BioReagent
assay   ≥99.7%
autoignition temp.   573 °F
expl. lim.   42 %, 63 °F
impurities   endotoxin, tested
refractive index   n20/D 1.479(lit.)
bp   189 °C(lit.)
mp   16-19 °C(lit.)
solubility   H2O: miscible (completely)
density   1.10 g/mL(lit.)
suitability   suitable for hybridoma

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

DMSO is a polar aprotic solvent used in chemical reactions, in polymerase chain reactions (PCR) and as a cryoprotectant vitrification agent for the preservation of cells, tissues and organs. DMSO is used in cell freezing media to protect cells from ice crystal induced mechanical injury. It is used for frozen storage of primary, sub-cultured, and recombinant heteroploid and hybridoma cell lines; embryonic stem cells (ESC), and hematopoietic stem cells. DMSO is frequently used in the combinations with BSA or fetal bovine serum (FBS).

Caution

Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.

Other Notes

This product is a Hybri-Max product. It is hybridoma tested and is assessed for suitability in cell freezing. This product is sterile filtered (0.2 micron) and tested for endotoxin levels.

Packaging

5 mL and 10 mL in flame sealed ampules, 100 mL in amber bottle

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Protocols & Applications

Protocol for cyropreservation

Price and Availability


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Safety & Documentation

Safety Information

RIDADR 
NA 1993 / PGIII
WGK Germany 
1
RTECS 
PV6210000
Flash Point(F) 
188.6 °F
Flash Point(C) 
87 °C
Protocols & Articles

Protocols

Protocol for the Cryopreservation of Cell Lines

This protocol is recommended by the European Collection of Cell Cultures (ECACC) for the cryopreservation of its cell lines. This protocol employs the use of passive methods involving an electric –80...

Resuscitation of Frozen Cell Lines

Many cultures obtained from a culture collection, such as ECACC, will arrive frozen and in order to use the cells they must be thawed and put into culture. It is vital to thaw cells correctly in orde...
Fundamental Techniques in Cell Culture Laboratory Handbook - 2nd Edition
Keywords: Centrifugation, PAGE

Related Content

Bio Brief: DMSO [VIDEO]

Sigma's Hybri-Max™ DMSO is supplied in single-use, flame-sealed ampules, eliminating the need for time-consuming filtration and preventing microbial contamination. This is the researcher-preferred DM...
Keywords: Cell culture, Cryopreservation, Filtration, Phase transitions

Peer-Reviewed Papers
15

References

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