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D2650 Sigma

Dimethyl sulfoxide

Hybri-Max, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%

Synonym: DMSO

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Properties

Related Categories BRDU Cell Proliferation Assay Reagents, Cell Biology, Cell Culture, Cell Freezing Media and Reagents, Cell Signaling and Neuroscience,
Quality Level   PREMIUM
grade   Hybri-Max
vapor density   2.7 (vs air)
vapor pressure   0.42 mmHg ( 20 °C)
InChI Key   IAZDPXIOMUYVGZ-UHFFFAOYSA-N
sterility   sterile-filtered
product line   BioReagent
assay   ≥99.7%
autoignition temp.   573 °F
expl. lim.   42 %, 63 °F
impurities   endotoxin, tested
refractive index   n20/D 1.479(lit.)
bp   189 °C(lit.)
mp   16-19 °C(lit.)
solubility   H2O: miscible (completely)
density   1.10 g/mL(lit.)
suitability   suitable for hybridoma

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

DMSO is a polar aprotic solvent used in chemical reactions, in polymerase chain reactions (PCR) and as a cryoprotectant vitrification agent for the preservation of cells, tissues and organs. DMSO is used in cell freezing media to protect cells from ice crystal induced mechanical injury. It is used for frozen storage of primary, sub-cultured, and recombinant heteroploid and hybridoma cell lines; embryonic stem cells (ESC), and hematopoietic stem cells. DMSO is frequently used in the combinations with BSA or fetal bovine serum (FBS).

Caution

Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.

Other Notes

This product is a Hybri-Max product. It is hybridoma tested and is assessed for suitability in cell freezing. This product is sterile filtered (0.2 micron) and tested for endotoxin levels.

Packaging

5 mL and 10 mL in flame sealed ampules, 100 mL in amber bottle

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Protocols & Applications

Protocol for cyropreservation

Price and Availability


Filter out uncertainty.

All labs need water
Safety & Documentation

Safety Information

RIDADR 
NA 1993 / PGIII
WGK Germany 
1
RTECS 
PV6210000
Flash Point(F) 
188.6 °F
Flash Point(C) 
87 °C

Frequently Asked Questions

What is the molarity of DMSO?
DMSO will have a molarity of 14.1M, based on a density of 1.1 g/mL and a molecular weight of 78.13 g/mol.
What is the DMSO dissolved in?
DMSO is not dissolved in any solvent. It is a pure chemical compound that is a liquid at room temperature.
Where does DMSO come from?
DMSO is known to have been isolated from tree bark in the past. It is now a commericially synthesized solvent.
What concentration of DMSO do I use in a cell freezing media?
DMSO is usually used at 1-10%, depending on the cell line.  For the cell freezing media that we offer, it is used at 8.7% concentration.
Does DMSO have any synthetic utility?
DMSO has been used to oxidize thiols and disulfides to sulfonic acids, hydrolysis of epoxides, thioalkylation of phenols, and oxidation of primary alcohols and halides, and esters of primary alcohols to aldehydes. To view this information in the D2650 product information sheet, click on the following link: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Product_Information_Sheet/d2650pis.Par.0001.File.tmp/d2650pis.pdf
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
What is the specification for 'pass' for cell freezing on the Certificate of Analysis for Dimethyl sulfoxide (DMSO)?
Product D2438 is assessed for cell freezing.  Cells are frozen and stored at -70°C.  The cells are thawed, cultured and viability is assessed at 24 hours.  There is no evidence of cytotoxicity or abnormal cell growth.
Dimethyl sulfoxide (DMSO) should be a liquid.  Why did it arrive as a solid?
DMSO supercools easily, and re-melts slowly at room temperature. The melting point is 16-19 °C. The solidified product can be re-liquefied by warming to room temperature without detriment to the product.
What is the basis for the endotoxin specification for Dimethyl sulfoxide (DMSO)?
The industry standard for 'endotoxin free' is less than 1 EU/mL.
What type of filter is used to sterile-filter Dimethyl sulfoxide (DMSO)?
DMSO can be sterile-filtered through a 0.2 micron PTFE filter. The sterility testing is per the current US Pharmacopoeia method.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
  What kind of solution is the A5955 - Antibiotic Antimycotic Solution (100×), Stabilized product suspended in?
A5955 - Antibiotic Antimycotic Solution (100×), Stabilized is supsended in a sodium citrate buffer solution. Source: Israel production group
Show more questions
Protocols & Articles

Articles

Accelerated Osteocyte Differentiation of Human Mesenchymal Stem Cells in a Novel Xeno-free and Serum-free Osteogenesis Differentiation Media

Mesenchymal stem cells (MSCs) have the capacity for multi-lineage differentiation, giving rise to a variety of mesenchymal phenotypes such as osteoblasts (bone), adipocytes (fat), and chondrocytes (c...
Anna Abai, Nick Asbrock and Vi Chu1
1 MilliporeSigma, Bioscience Division, Temecula, CA, USA
 
Keywords: Cell culture, Clinical, Drug discovery, Gene expression, Immunostaining

Protocols

Mesenchymal Stem Cell Culture Protocols

Introduction Methods   Isolation of Mesenchymal Stem Cells   Expansion of Human Mesenchymal Stem Cells     Preparation of Coated Plates     Thawing of Mesenchymal Stem Cells     Expansion of Mesenchy...
Keywords: Cell disruption, Clinical, Culture media, Gene expression, Immunocytochemistry, Stem cell biology

Protocol for the Cryopreservation of Cell Lines

This protocol is recommended by the European Collection of Authenticated Cell Cultures (ECACC, a part of Public Health England) for the cryopreservation of its cell lines. This protocol employs the u...
Keywords: Cryopreservation, Phase transitions

Resuscitation of Frozen Cell Lines

Many cultures obtained from a culture collection, such as ECACC, will arrive frozen and in order to use the cells they must be thawed and put into culture. It is vital to thaw cells correctly in orde...
Fundamental Techniques in Cell Culture Laboratory Handbook - 2nd Edition
Keywords: Centrifugation, PAGE

Related Content

Bio Brief: DMSO [VIDEO]

Sigma's Hybri-Max™ DMSO is supplied in single-use, flame-sealed ampules, eliminating the need for time-consuming filtration and preventing microbial contamination. This is the researcher-preferred DM...
Keywords: Cell culture, Cryopreservation, Filtration, Phase transitions

Peer-Reviewed Papers
15

References

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