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D2812 Sigma

REDTaq® Genomic DNA Polymerase

without MgCl2

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Properties

Related Categories Application Index, Biochemicals and Reagents, Core Bioreagents, Enzymes, Inhibitors, and Substrates, General Reagents,
form   liquid
feature   hotstart: No
concentration   1 unit/μL
color   red
Featured Industry   Agriculture
shipped in   wet ice
storage temp.   −20°C

Description

Components

Includes 10× PCR reaction buffer without MgCl2 and a separate tube of 25 mM MgCl2

Application

For routine genomic DNA amplifications

Features and Benefits

• Enhanced amplification on genomic and difficult DNA templates
• Same great performance as Taq DNA Polymerase in a more convenient format for high throughput applications
• Quick recognition and confirmation of appropriate mixing
• No loading buffers or tracking dyes necessary. Sample can be taken directly from reaction and loaded onto an agarose gel

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.

REDTaq is a registered trademark of Sigma-Aldrich Co. LLC

Unit Definition

One unit incorporates 10 nmol of total dNTPs into acid precipitable DNA in 30 min. at 74°C.

General description

REDTaq Genomic DNA Polymerase is a unique blend of Taq DNA Polymerase with an inert red dye. This special formulation is designed to provide enhanced amplification of more complex or genomic templates. REDTaq Genomic DNA Polymerase is highly sensitive, produces increased yields and is capable of generating longer product lengths. It has all the advantages of REDTaq DNA polymerase, such as easy visualization of enzyme addition and complete reaction mixing, and direct loading to an agarose gel. The dye migrates slightly faster than bromophenol blue at approximately the same rate as a 125 base pair fragment.
The inert red dye does not effect automated or manual sequencing, restriction digestions or other downstream applications. The dye can easily removed by any standard purification method.

Price and Availability


Ultrapure dNTPs for PCR

Kapa Biosystems
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

Improving Real-Time PCR Success

To meet increasing food supply demands of the global population, growers require seeds that can withstand the regional challenges found in their fields. In order to develop crop varieties that are mo...
Keywords: Agriculture, Amplification, Environmental, Polymerase chain reaction

Protocols

Amplification of Genomic DNA using REDTaq DNA Polymerase

REDTaq DNA Polymerase is a convenient package that includes all the necessary components for a PCR reaction except primers, DNA template and water. The formulation has been optimized for amplificatio...
Keywords: AGE, Amplification, Applications, Buffers, Digestions, Electrophoresis, Evaporation, Formulations, Gel electrophoresis, Polymerase chain reaction, Purification, Sequencing, transformation

Related Content

Agriculture | Crop Protection Workflow

Pesticides are an essential component in modern agriculture and enable growers to fully realize the yield potential of their seeds. From compound discovery to active ingredient development, our chemi...
Keywords: Agriculture, Pesticides

Agriculture | Genotyping

Sigma-Aldrich’s chemistry expertise can help customers develop and optimize their leaf and seed genotyping workflow to reduce cost per data point while increasing throughput rates. In addition, our g...
Keywords: Amplification, Polymerase chain reaction, Polymerase chain reaction - quantitative, Sequencing, Whole genome amplification

Agriculture | Plant Breeding Workflow

Sigma-Aldrich products are aligned to the discovery, development and production phases of the plant breeding workflow, allowing you to develop and produce new crop varieties faster. With materials ma...

PCR Selection Guide

Sigma-Aldrich offers a wide variety of PCR reagents to meet any experimental needs. Our range of polymerases is customized to meet your End-Point PCR, qPCR, or RT-PCR needs. Our products vary from ro...
Keywords: Polymerase chain reaction, Polymerase chain reaction - quantitative

Peer-Reviewed Papers
15

References

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Related Products

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Product #

Description

Add to Cart

D6063 REDTaq® SuperPak DNA Polymerase, Taq for routine PCR with inert dye; with 10X buffer & dNTP mix
D8312 REDTaq® Genomic DNA Polymerase, with MgCl2
06693 Timestrip Plus -20 °C

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