Lambda DNA Mixed Digest
buffered aqueous solution
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EC Number 231-791-2
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Popular Documents: Datasheet (PDF) | Specification Sheet (PDF)
Properties
| Related Categories | DNA Digest Markers, Molecular Biology, Nucleic Acid Electrophoresis, Nucleic Acid Markers |
| form | buffered aqueous solution |
| storage temp. | −20°C |
Description
Other Notes
Contains 10 fragments, 1,503–48,502 bp
Reconstitution
A sample of the marker should be diluted with gel loading buffer to an appropriate concentration, typically 0.2 μg/μL. Loading 2 μg per well (10 μL) is sufficient to be seen using ethidium bromide staining.
Physical form
Solution in 10 mM Tris-HCl, pH 8.0, 1 mM EDTA
Application
DNA gel electrophoresis marker. Contains uncut bacteriophage λ plus λ that has been completely digested by Apa I, Kpn I, Xba I and Xho I in separate reactions. Sticky ends have been filled in using DNA polymerase I Klenow fragment to prevent reannealing. The recommended agarose gel concentration is 0.4% for this marker.
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