|Related Categories||DNA Digest Markers, Molecular Biology, Nucleic Acid Electrophoresis, Nucleic Acid Markers|
|form||buffered aqueous solution|
Contains 10 fragments, 1,503–48,502 bp
A sample of the marker should be diluted with gel loading buffer to an appropriate concentration, typically 0.2 μg/μL. Loading 2 μg per well (10 μL) is sufficient to be seen using ethidium bromide staining.
Solution in 10 mM Tris-HCl, pH 8.0, 1 mM EDTA
DNA gel electrophoresis marker. Contains uncut bacteriophage λ plus λ that has been completely digested by Apa I, Kpn I, Xba I and Xho I in separate reactions. Sticky ends have been filled in using DNA polymerase I Klenow fragment to prevent reannealing. The recommended agarose gel concentration is 0.4% for this marker.
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