|Related Categories||DNA Digest Markers, Molecular Biology, Nucleic Acid Electrophoresis, Nucleic Acid Markers|
|grade||for molecular biology|
|form||buffered aqueous solution|
|usage||10 uses (20 μg size)|
|50 uses (0.1 mg size)|
|suitability||suitable for electrophoresis (DNA)|
|shipped in||dry ice|
Sigma’s Lambda DNA Mixed Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
Sigma’s Lambda DNA Mixed Digest contains 10 fragments from 1,503 − 48,502 bp. Six ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.
For optimal resolution, the recommended agarose gel concentration is 0.4%.
Bacteriophage lambda DNA was completely digested with by Apa I, Kpn I, Xba I and Xho I in separate reactions, sticky ends filled with DNA polymerase I Klenow (to prevent reannealing), mixed with uncut bacteriophage lambda DNA, and then purified by phenol extraction.
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DNA and RNA size markers contain a mixture of DNA (or RNA) fragments of known length, making them suitable for estimating the fragment length of concurrently run samples. They stain well with ethidiu...
Keywords: AGE, Electrophoresis, Gel electrophoresis, PAGE, Polymerase chain reaction
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