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D3035 Sigma

Deoxyribonucleic acid from human placenta

buffered aqueous solution, sexed, female

Synonym: DNA

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Properties

Related Categories DNA, Mammalian DNA, Molecular Biology, Molecular Biology Reagents
grade   for molecular biology
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C

Description

Application

For use in Southern hybridizations.

Deoxyribonucleic acid from human placenta has been used:
• to compare the efficiency of DNA quantification methods
• as a standard in real-time PCR analysis of DNA samples from bladder cancer cell lines
• as an internal control to estimate the degree of fragmentation of circulating DNA
• for restriction digest analysis to identify low copy repeat regions of human chromosome 22q11
The product is suitable for use as a blocking agent in Southern hybridizations.

General description

Human placental DNA is isolated from a single female donor placenta, but will contain some maternal DNA. The sex has been determined using hybridization with DNA probes.

Recommended products

To prevent shearing of the large genomic DNA, this product should be dissolved in water at 1 mg/ml with no sonication or stirring. Gentle inversion overnight at 0 - 4 C is recommended to completely solubilize the DNA. The presence of 1 mM EDTA is recommended to prevent nucleases from degrading the DNA.


Solutions of DNA have been stored successfully for several months at 4 C, in 10 mM Tris, pH 7.5 - 8.0, with 1 mM EDTA and without a bacteriostatic agent. At low concentrations, about 25 μg/ml, DNA tends to absorb onto the surfaces of plastic tubes.

Price and Availability


Laboratory Gloves

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
HG1933000

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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