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D3287 Sigma

Deoxyribonucleic acid, single stranded from human placenta

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Properties

Related Categories Core Bioreagents, DNA, DNA for hybridization, General Reagents, Life Science Reagents for Northern and Southern Blotting,
grade   for molecular biology
description   For hybridization
form   solution
mol wt   mol wt (Fragments from 587-831 bp.)
solubility   water: soluble9 - 12 mg/mL
storage temp.   −20°C

Description

Components

DNA is supplied in a solution of 100mM phosphate buffer.  This is a ready to use concentrated solution
of 9-12 mg/ml DNA in 100mM phosphate buffer. However, it will reanneal on standing at room temperature so it is recommended to boil the solution for 10 minutes and then cool on ice for at least 5 minutes prior to use. Cooling on ice will
reduce the chances for reannealing, as it is more likely to reanneal if cooled at room temperature.

Application

Many factors contribute to the signal-to-noise ratio in nucleic acid hybridizations. These factors include the presence of solvent (formamide), hybridization temperature, length of hybridization, volume of hybridization solution, degree and method of agitation, use of blocking reagents, concentration and specific activity of the probe, use of molecular agents to increase the rate of nucleic acid reassociation, and the degree of stringency used during the washing of the membrane.

In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. Generally, a combination of blocking reagent, detergent, and denatured, fragmented DNA is used to accomplish this. Sigma offers sonicated, denatured DNA from a variety of species for use as a blocking agent in Northern and Southern blotting and other nucleic acid hybridization techniques.

Sonicated Deoxyribonucleic acid, single stranded from human placenta, was used as blocking agent in Southern hybridization of DNA from human papillomavirus (HPV) positive SiHa, HeLa and CaSki cell-lines. It was used as standard in GC/MS analysis of exocyclic DNA adducts.

Features and Benefits

• High quality human DNA.
• DNA fragments of defined sizes.

General description

Human placental DNA is isolated from donor placenta, but will contain some maternal DNA. The DNA fragments are sonicated to produce fragments of consistent size.

Packaging

5×1 mL in glass bottle

Price and Availability


Laboratory Gloves

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
HG1933000

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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Product #

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