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  • D3438 - Anti-DFF45, N-Terminal antibody produced in rabbit

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D3438 Sigma

Anti-DFF45, N-Terminal antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-ICAD

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies to DNA Fragmentation,
species reactivity   human
application(s)   western blot: 0.5 μg/mL using human HeLa cell extract
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt 45 kDa (non-apoptotic cells)
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... DFFA(1676)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 2-21 of the N-terminal region of human DFF45/ICAD (DNA Fragmentation Factor 45/Inhibitor of Caspase-3 Activated DNase I).

General description

DFF exists as an inactive cytoplasmic protein until activated by apoptotic signals. DFF40, the nuclease responsible for DNA fragmentation during apoptosis, exists as a heterodimeric complex with DFF45.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.

Application

Anti-DFF45 antibody produced in rabbit is suitable for immunoblotting at a working concentration of 0.5μg/mL using a total HeLa cell lysate.

Features and Benefits

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Biochem/physiol Actions

DFF45 functions as both a chaperone, mediating the correct folding of DFF40, and an inhibitor of DFF40. DFF45 is cleaved by caspase-3 at two sites in response to apoptotic signals. This releases DFF40, the active nuclease. DFF40 seems to oligomerize, forming a large, functional complex which breaks down DNA by introducing double strand breaks. Also, DFF40 appears to interact directly with histone H1 that may stimulate its activity.

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