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D3570 Sigma

Anti-DVL1 antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution, antigen mol wt ~85 kDa

Synonym: Anti-Dishevelled-1, Anti-Dsh homolog 1, Anti-Segment polarity protein dishevelled homolog

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Neurobiology, Antibodies to Wnt Signaling, DQ-DZ,
species reactivity   human
application(s)   western blot: 1-2 μg/mL using HEK-293T cell lysate expressing human DVL1
clone   polyclonal
concentration   ~1.5 mg/mL
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt ~85 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... DVL1(1855)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 552-567 of human DVL1, conjugated to KLH. The corresponding sequence is conserved (81% identity) in rat and mouse DVL1.

General description

The three DVL isoforms display high sequence homology and have conserved DIX, PDZ and DEP domains required for GSK3β inactivation. DVL1 is highly expressed in adult and fetal tissues such as skeletal muscle and pancreas but is also found in the brain and neural tube.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Anti-DVL1 antibody produced in rabbit is suitable for western blot analysis at a working concentration of 1-2μg/mL using HEK-293T cell lysate expressing human DVL1. It was used as a primary antibody at a working dilution of 1:3000 in the immunohistochemical staining of tissue sections of ganglionic and aganglionic colon segments to understand the expression of dishevelled gene in Hirschsprung’s disease.

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Biochem/physiol Actions

Dishevelled (Dsh, DVL) proteins are part of a multigene family that mediate wnt signaling pathways that are essential for the regulation of cellular proliferation, differentiation, motility, and morphogenesis. DVL1 regulates the activity of JNK (c-Jun N-terminal kinase) and GSK3β in the wnt signaling pathway. Upon activation of the wnt signaling pathway, DVL1 inactivates GSK3β through complex formation with APC (Antigen-presenting cell), β-catenin and axin proteins, releasing β-catenin from degradation. In neurons, DVL1 is localized to axonal microtubules and stabilizes microtubules by inhibiting the GSK3β mediated phosphorylation of MAP-1B.

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  1
Protocols & Articles

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Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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