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D3764 Sigma

DEAE-Cellulose

preswollen, microgranular

Synonym: Cellulose DEAE, Diethylaminoethyl-cellulose

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Properties

Related Categories Anion Exchange Media, Ion Exchange Chromatography, Molecular Biology, Protein Chromatography, Proteomics More...
form   preswollen, microgranular
contains   ~70% moisture content
capacity   ~0.3 meq/g(as supplied)
  ~1.0 meq/g(dry)

Description

Application

DEAE-Cellulose is used in ion exchange chromatography, protein chromatography and anion exchange media. DEAE cellulose has been used to study acute myocardial infarction

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Safety Information

WGK Germany 
3

Protocols & Articles

Peer-Reviewed Papers

References

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Reversed-phase LC/MS method for polyphosphoinositide analyses: changes in molecular species levels during epidermal growth factor activation in A431 cells. Ogiso H and Taguchi R Anal. Chem. 80(23), 9226-32, (2008)

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Development of a reverse-phase liquid chromatography electrospray ionization mass spectrometry method for lipidomics, improving detection of phosphatidic acid and phosphatidylserine. Ogiso H, Suzuki T, and Taguchi R Anal. Biochem. 375(1), 124-31, (2008)

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Enzymatic route to preparative-scale synthesis of UDP-GlcNAc/GalNAc, their analogues and GDP-fucose. Zhao, G., et al. Nat. Protoc. 5(4), 636-646, (2010)

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Isoforms of acyl carrier protein involved in seed-specific fatty acid synthesis. Suh, M.C., et al. Plant J. 17(6), 679-688, (1999)

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Purification and characterisation of lignin peroxidase from Pycnoporus sanguineus MTCC-137. Sharma, J.K., et al. Prikl. Biokhim. Mikrobiol. 47, 584-589, (2011)

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Impact of ligand density on the optimization of ion-exchange membrane chromatography for viral vector purification. Vicente T, Fáber R, Alves PM, et al. Biotechnol. Bioeng. 108(6), 1347-59, (2011)

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High-resolution mapping of points of site-specific replication stalling. Vengrova S and Dalgaard JZ Methods Mol. Biol. 521, 215-27, (2009)

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Purification of restriction fragments containing replication intermediates from complex genomes for 2-D gel analysis. Mesner LD, Dijkwel PA, and Hamlin JL Methods Mol. Biol. 521, 121-37, (2009)

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Replication initiation point mapping: approach and implications. Das-Bradoo S and Bielinsky AK Methods Mol. Biol. 521, 105-20, (2009)

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Decontamination of polymerase chain reaction reagents using DEAE-cellulose. Glushkov SA, Bragin AG, and Dymshits GM Anal. Biochem. 393(1), 135-7, (2009)

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Characterisation of a haemagglutinin from Hokkaido red bean (Phaseolus vulgaris cv. Hokkaido red bean). Wong JH, Wan CT, and Ng TB J. Sci. Food Agric. 90(1), 70-7, (2010)

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Multiple replication origins of Halobacterium sp. strain NRC-1: properties of the conserved orc7-dependent oriC1. James A. Coker et al J. Bacteriol. 191, 5253-61, (2009)

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A hemagglutinin with mitogenic activity from dark red kidney beans. Xia L and Ng TB J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci. 844(2), 213-6, (2006)

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Probing the active site tightness of DNA polymerase in subangstrom increments. Kim, T.W., et al. Proc. Natl. Acad. Sci. U. S. A. 102(44), 15803-15808, (2005)

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Ni speciation in tea infusions by monolithic chromatography--ICP-MS and Q-TOF-MS. Ščančar J, Zuliani T, Žigon D, et al. Anal. Bioanal. Chem 405(6), 2041-51, (2013)

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Potential applications of immobilized bitter gourd (Momordica charantia) peroxidase in the removal of phenols from polluted water. Akhtar S and Husain Q Chemosphere 65(7), 1228-35, (2006)

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A peptide with potent antifungal and antiproliferative activities from Nepalese large red beans. Ma DZ, Wang HX, and Ng TB Peptides 30(12), 2089-94, (2009)

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A Drosophila Homolog of Bovine smg p25a GDP Dissociation Inhibitor Undergoes a Shift in Isoelectric Point in the Developmental Mutant quartet Zahner, J., and Cheney, C. Mol. Cell. Biol. 13, 217-227, (1993)

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Generation of chemically engineered ribosomes for atomic mutagenesis studies on protein biosynthesis. Erlacher, M.D., et al. Nat. Protoc. 6, 580-592, (2011)

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Cryopreservation of Trypanosoma evansi after DEAE-cellulose purification: Evaluation of infective parameters. Tavares KC, Da Silva AS, Wolkmer P, et al. Res. Vet. Sci. 90(2), 257-9, (2011)

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COMP (Cartilage Oligomeric Matrix Protein) is Synthesized in Ligament, Tendon, Meniscus, and Articular Cartilage. Müller, G., et al. Connect. Tissue Res. 39(4), 233-244, (1998)

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Tentacle carrier for immobilization of potato phenoloxidase and its application for halogenophenols removal from aqueous solutions. Lončar N and Vujčić Z J. Hazard. Mater. 196, 73-8, (2011)

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Metal distribution and metallothionein induction after cadmium exposure in the terrestrial snail Helix aspersa (Gastropoda, Pulmonata). Hispard F, Schuler D, de Vaufleury A, et al. Environ. Toxicol. Chem. 27(7), 1533-42, (2008)

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Interaction of cellulose-based cationic polyelectrolytes with mucin. Mazoniene E, Joceviciute S, Kazlauske J, et al. Colloids Surf. B Biointerfaces 83(1), 160-4, (2011)

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Highly thermostable xylanase of the thermophilic fungus Talaromyces thermophilus: purification and characterization. Maalej I, Belhaj I, Masmoudi NF, et al. Appl. Biochem. Biotechnol. 158(1), 200-12, (2009)

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Isolation and characterization of novel protein with anti-fungal and anti-inflammatory properties from Aloe vera leaf gel. Das S, Mishra B, Gill K, et al. Int. J. Biol. Macromol. 48(1), 38-43, (2011)

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Soluble tubulin complexes, gamma-tubulin, and their changing distribution in the zebrafish (Danio rerio) ovary, oocyte and embryo. Liu J and Lessman CA Comp. Biochem. Physiol. B Biochem. Mol. Biol. 147(1), 56-73, (2007)

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Striped mullet (Mugil cephalus) hemoglobin system: multiplicity and functional properties. Olianas A, Meloni C, Messana I, et al. J. Comp. Physiol. B, Biochem. Syst. Environ. Physiol. 181(2), 187-97, (2011)

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Purification of matrix metalloproteinases by column chromatography. Imai K and Okada Y Nat. Protoc. 3(7), 1111-24, (2008)

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Development of magnetically separable immobilized lipase by using cellulose derivatives and their application in enantioselective esterification of ibuprofen. Lee G, Joo H, Kim J, et al. J. Microbiol. Biotechnol. 18(3), 465-71, (2008)

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Novel carbohydrate-binding activity of bovine liver beta-glucuronidase toward lactose/N-acetyllactosamine sequences. Matsushita-Oikawa H, Komatsu M, Iida-Tanaka N, et al. Glycobiology 16(10), 891-901, (2006)

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Immobilization of phenylalanine dehydrogenase and its application in flow-injection analysis system for determination of plasma phenylalanine. Tarhan L and Ayar-Kayali H Appl. Biochem. Biotechnol. 163(2), 258-67, (2011)

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Robust physical methods that enrich genomic regions identical by descent for linkage studies: confirmation of a locus for osteogenesis imperfecta. Peter Brooks et al BMC Genet. 10, 16, (2009)

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Affinity-purification of fibrinogenase with high proteolytic activity from Agkistrodon halys (Chinese) Venom. Ma B, Zhang Y, Wu D, et al. Arch. Pharm. Res. 31(9), 1129-36, (2008)

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Conformational free energies of myoglobins of small mammals. Kelly, L., et al. Int. J. Pept. Protein Res. 31(3), 281-288, (1988)

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Quantitative determination of nucleosides and their phosphate esters - 1. The acidic nucleosides, 3-deazauridine and 6-azauridine. Welch, A.D., et al. Int. J. Biochem. 16(6), 587-591, (1984)

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Rescued human virus (RHV): Association with melanoma. Závada, J., et al. Int. J. Cancer 42(4), 572-575, (1988)

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Structure elucidation and antioxidant activity of a novel polysaccharide isolated from Boletus speciosus Forst. Ding, X., et al. Int. J. Biol. Macromol. 50(3), 613-618, (2012)

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Isolation and study of insulin activated nitric oxide synthase inhibitory protein in acute myocardial infarction subjects. Ray, U., et al. J. Thromb. Thrombolysis 33(3), 218-229, (2012)

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