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D3937 Sigma

DirectLoad 1 kb DNA Ladder

ready-to-use marker for DNA electrophoresis

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Properties

Related Categories Core Bioreagents, DNA Ladder Markers, Life Science Reagents for DNA/RNA Electrophoresis, Markers and Ladders, Molecular Biology,
form   liquid
usage   100 uses
suitability   suitable for electrophoresis (DNA)
storage temp.   −20°C

Description

Components

Sigma’s DirectLoad 1kb Ladder is provided in a gel-loading solution of 2.5% Ficoll (Type 400), 0.0125% bromophenol blue, and 0.00625% xylene cyanol.

Application

Suitable for size determination of dsDNA by DNA electrophoresis with either agarose or polyacrylamide gels.

Features and Benefits

• Ready-to-load
• Easy-to-use
• Popular band sizes

General description

Sigma’s DirectLoad 1 kb Ladder contains 11 fragments consisting of 500 bp repeats from 0.5 to 3 kb, 1 kb repeats from 3 to 6 kb, and 2 kb repeats from 6 to 10 kb. For NA electrophoresis, 5 ul of the marker can be loaded directly into a single lane on an agarose or polyacrylamide gel. Suitable for use in Northern and Southern blotting.

Other Notes

Contains 11 fragments consisting of 500 bp repeats from 0.5 to 3 kb, 1 kb repeats from 3 to 6 kb and 2 kb repeats from 6 to 10 kb.

For optimal resolution, the recommended agarose gel concentration is 0.75%.

Legal Information

DirectLoad is a trademark of Sigma-Aldrich Co. LLC

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Introduction to Markers and Ladders

Sigma-Aldrich offers a variety of markers that aid size determination of samples separated by agarose and/or polyacrylamide gel electrophoresis. These products include markers for DNA, PCR fragments ...
Keywords: Digestions, Electrophoresis, Gel electrophoresis, PAGE, Polymerase chain reaction

Introduction to Nucleic Acid Electrophoresis

Introduction Agarose gel electrophoresis for DNA Agarose gel electrophoresis for RNA Polyacrylamide gel electrophoresis for DNA Reference
Keywords: AGE, Buffers, Degradations, Electrophoresis, Environmental, Gel electrophoresis, PAGE, Purification, Separation, Size-exclusion chromatography

Markers and Ladders Selection Guide

DNA and RNA size markers contain a mixture of DNA (or RNA) fragments of known length, making them suitable for estimating the fragment length of concurrently run samples. They stain well with ethidiu...
Keywords: AGE, Electrophoresis, Gel electrophoresis, PAGE, Polymerase chain reaction

Protocols

Antibody-Enzyme Mediated Hot Start PCR Protocol

During PCR assay preparation, nonspecific amplification can occur due to binding of PCR primers to nonspecific templates and from formation of primer dimers which result from using other primer molec...
Keywords: AGE, Amplification, Buffers, Electrophoresis, Enzyme activity, Gel electrophoresis, Nucleic acid denaturation, Polymerase chain reaction, Size-exclusion chromatography

End Point PCR Protocol for Long and Accurate DNA Amplification

Technology Overview Equipment Reagents Assay Considerations Procedure Troubleshooting Materials References

Hot Start Taq Polymerase Protocol to Reduce Non-Specific Amplification

As PCR reactions sit at room temperature, during assay setup, nonspecific amplification can occur via:
Keywords: AGE, Amplification, Electrophoresis, Gel electrophoresis, Gene expression, Nucleic acid annealing, Polymerase chain reaction, Size-exclusion chromatography

Related Content

Bionic™ Buffer Data

Bionic Buffer is a unique alternative to traditional TBE (TRIS borate-EDTA) and TAE (TRIS acetate-EDTA) electrophoresis buffers. Bionic Buffer allows for:
Keywords: Buffers, Electrophoresis, Polymerase chain reaction, Separation

Peer-Reviewed Papers
15

References

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