|Related Categories||Alphabetical Listing, Antibodies, Biochemicals and Reagents, Blotting/Histochemistry Substrates, Colorimetric,|
Developed for use in immunohistology and immunoblotting as a precipitating substrate for the localization of peroxidase activity. DAB is the immunohistology substrate of choice as it produces an intense brown-black stain which is resistant to alcohol. Slides stained with DAB may be dehydrated, mounted in resinous media, and stored for future reference. SIGMAFAST™ DAB Tablets require no additional buffers or steps to prepare an active substrate solution. Tablets are individually packaged in foil packets
Each tablet set dissolved in 1 mL deionized water yields a ready-to-use buffered solution containing DAB and urea hydrogen peroxide.
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
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tablet, 10 mg substrate per tablet
tablet, To prepare 5 mL
tablet, To prepare 15 mL
Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrat...
BioFiles 2008, 3.4, 6.
Keywords: Enzyme-linked immunosorbent assay, Immunohistochemistry, Western blot
As a leading supplier of antibodies, Sigma® Life Science is committed to providing researchers the antibody they need with the quality they deserve. Our Antibody Explorer Search Tool provides easy ac...
Keywords: Buffers, Cell biology, Epigenetics, Immunohistochemistry, Immunoprecipitation, Molecular biology, Neuroscience, Western blot
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