SIGMAFAST™ 3,3′-Diaminobenzidine tablets tablet, To prepare 1 mL

Properties

Related Categories	Alphabetical Listing, Antibodies, Biochemicals and Reagents, Blotting/Histochemistry Substrates, Colorimetric, Core Bioreagents, Detection, Detection Substrates, Enzyme Substrates, Enzymes, Inhibitors, and Substrates, Immunohistochemistry, Life Science Reagents for Immunohistochemistry (IHC), Life Science Reagents for Western Blotting, Peroxidase, Peroxidase Substrates, Plant Biotechnology, Plant Proteomics, Protein Detection, Research Essentials, Substrates, Substrates by Enzyme, Substrates for Peroxidase, Substrates, Buffers & Blockers, Supplementary Products, Tablets, Western Blotting, Western Blotting Reagents More...
form	tablet
storage temp.	−20°C

Description

Application

Developed for use in immunohistology and immunoblotting as a precipitating substrate for the localization of peroxidase activity. DAB is the immunohistology substrate of choice as it produces an intense brown-black stain which is resistant to alcohol. Slides stained with DAB may be dehydrated, mounted in resinous media, and stored for future reference. SIGMAFAST^™ DAB Tablets require no additional buffers or steps to prepare an active substrate solution. Tablets are individually packaged in foil packets

Reconstitution

Each tablet set dissolved in 1 mL deionized water yields a ready-to-use buffered solution containing DAB and urea hydrogen peroxide.

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Price and Availability

Related Papers

Premature differentiation and aberrant movement of pituitary cells lacking both Hes1 and Prop1. Ashley D. Himes et al Dev. Biol. 325, 151-61, (2009)

Documents

Certificate of Analysis

D4168 - Datasheet (91 KB)

Safety Information

Hazard Codes (Europe)	Xn
Risk Statements (Europe)	22-36/37/38
Safety Statements (Europe)	26-36/37

Technical information & documentation associated with this product is available in the Safety & Documentation tab.

Articles

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrat...
BioFiles 2008, 3.4, 6.
Keywords: Enzyme-linked immunosorbent assay, Immunohistochemistry, Western blot