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D4184 Sigma

JumpStart Taq DNA Polymerase

without MgCl2

Purchase

Properties

Related Categories Core Bioreagents, Hot Start PCR, Life Science Reagents for PCR, Molecular Biology, PCR Enzymes,
form   liquid
feature   hotstart
concentration   2.5 units/μL
color   colorless
  colorless
Featured Industry   Agriculture
shipped in   wet ice
storage temp.   −20°C

Description

Application

• For PCR amplifications that require reduced non-specific amplification
• For multiplex PCR
• For reduction of primer dimers

JumpStart® Taq DNA Polymerase is used for real time reverse-transcription polymerase chain reaction analysis (Real-Time PCR) in the following ways:
• Used for generation of complementary DNAs
• Genomic DNA isolation
• Isolation of total RNA from cultured cells
• IL-4R and IL-13R analysis

Other Notes

Sigma′s JumpStart Taq DNA Polymerase is an antibody-inactivated hot-start enzyme designed to minimize non-specific amplification while increasing target yield. Once the reaction temperature reaches 70°C, Taq DNA polymerase activity is restored and the resulting PCR exhibits a higher specificity and yield. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques. The enzyme may also be included in the master mix preparation resulting in more consistency from one reaction to the next.

View more detailed information on JumpStart Taq enzymes at www.sigma-aldrich.com/hotstart.

Packaging

JumpStart Taq DNA Polymerase is provided with a 10× reaction buffer available with and without MgCl2. The magnesium free 10× buffer also includes a separate tube of 25 mM MgCl2 for optimization.

Supplied with 10× reaction buffer without MgCl2. Includes a separate tube of 25 mM MgCl2

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.

Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.

Jacobs is a registered trademark of Jacobs

JumpStart is a trademark of Sigma-Aldrich Co. LLC

Unit Definition

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.

Features and Benefits

• Reduces non-specific amplification
• Increases PCR specificity and yield
• Reduces set-up time concerns associated with manual or wax Hot Start methods
• Activation time of less than 1 minute

Price and Availability


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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

Improving Real-Time PCR Success

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Introduction & Historical Timelines - PCR Technologies Guide

The Polymerase Chain Reaction (PCR) is used in all areas of biological science research, including the clinical, forensic and diagnostic fields and the widespread adoption of the PCR technique has re...
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Protocols

Amplification of DNA Using Jumpstart™ Taq DNA Polymerase

Note: JumpStart Taq DNA polymerase has been shown to work effectively with up to 5% v/v DMSO. Other co-solvents, solutes (e.g., salts) and extremes in pH or other reaction conditions may reduce the a...
Keywords: AGE, Amplification, Electrophoresis, Evaporation, Gel electrophoresis, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Reductions, Size-exclusion chromatography, Solvents, Titrations

Antibody-Enzyme Mediated Hot Start PCR Protocol

During PCR assay preparation, nonspecific amplification can occur due to binding of PCR primers to nonspecific templates and from formation of primer dimers which result from using other primer molec...
Keywords: AGE, Amplification, Buffers, Electrophoresis, Enzyme activity, Gel electrophoresis, Nucleic acid denaturation, Polymerase chain reaction, Size-exclusion chromatography

Hot Start Taq Polymerase Protocol to Reduce Non-Specific Amplification

As PCR reactions sit at room temperature, during assay setup, nonspecific amplification can occur via:
Keywords: AGE, Amplification, Electrophoresis, Gel electrophoresis, Gene expression, Nucleic acid annealing, Polymerase chain reaction, Size-exclusion chromatography

Related Content

Agriculture | Genotyping

Sigma-Aldrich’s chemistry expertise can help customers develop and optimize their leaf and seed genotyping workflow to reduce cost per data point while increasing throughput rates. In addition, our g...
Keywords: Amplification, Polymerase chain reaction, Polymerase chain reaction - quantitative, Sequencing, Whole genome amplification

PCR Selection Guide

Sigma-Aldrich offers a wide variety of PCR reagents to meet any experimental needs. Our range of polymerases is customized to meet your End-Point PCR, qPCR, or RT-PCR needs. Our products vary from ro...
Keywords: Polymerase chain reaction, Polymerase chain reaction - quantitative

Plant Breeding Workflow - Collaborating to Empower Yield Improvements

Sigma-Aldrich products are aligned to the discovery, development and production phases of the plant breeding workflow, allowing you to develop and produce new crop varieties faster. With materials ma...

Peer-Reviewed Papers
15

References

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P2893 JumpStart Taq ReadyMix, Complete optimized reagent for hot-start PCR at 2X concentration
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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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