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D4309 Sigma

REDTaq® DNA Polymerase

Taq for routine PCR with inert dye, 10X buffer included



Related Categories Application Index, Biochemicals and Reagents, Core Bioreagents, Enzymes, Inhibitors, and Substrates, Life Science Reagents for PCR,
form   liquid
feature   hotstart: no
concentration   1 unit/μL
color   red
Featured Industry   Agriculture
shipped in   wet ice
storage temp.   −20°C



For routine PCR amplifications

Features and Benefits

• Same great performance as Taq DNA Polymerase in a more convenient format for high throughput applications.
• Visual confirmation that not only has the enzyme been added, but that proper mixing has occurred.
• No additional loading dyes are necessary. An aliquot can be taken directly from the reaction and loaded onto an agarose gel for electrophoresis.


Provided with 10X reaction buffer containing MgCl2

Unit Definition

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.

General description

REDTaq DNA Polymerase is a unique blend of Sigma′s quality Taq DNA Polymerase combined with an inert red dye. This dye enables quick visual confirmation of enzyme addition and reaction mixing. An aliquot of the samples (5-10 μl) can then be loaded directly onto an agarose gel for electrophoresis following PCR. The red dye migrates slightly faster than bromophenol blue at about the same rate as a 125 base pair fragment in a 1% agarose gel. Since no additional loading buffers are added to the reaction following PCR, reamplification is possible.
The red dye has no effect on automated or manual sequencing, restriction digestions or other downstream applications. However, if removing the dye is desired, this can easily be accomplished using any standard purification method.

Legal Information

REDTaq is a registered trademark of Sigma-Aldrich Co. LLC

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.

Price and Availability

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Safety & Documentation

Safety Information

NONH for all modes of transport
Protocols & Articles


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Applications with REDTaq

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