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• Same great performance as Taq DNA Polymerase in a more convenient format for high throughput applications.
• Visual confirmation that not only has the enzyme been added, but that proper mixing has occurred.
• No additional loading dyes are necessary. An aliquot can be taken directly from the reaction and loaded onto an agarose gel for electrophoresis.
Provided with 10X reaction buffer containing MgCl2
One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.
REDTaq DNA Polymerase is a unique blend of Sigma′s quality Taq DNA Polymerase combined with an inert red dye. This dye enables quick visual confirmation of enzyme addition and reaction mixing. An aliquot of the samples (5-10 μl) can then be loaded directly onto an agarose gel for electrophoresis following PCR. The red dye migrates slightly faster than bromophenol blue at about the same rate as a 125 base pair fragment in a 1% agarose gel. Since no additional loading buffers are added to the reaction following PCR, reamplification is possible.
The red dye has no effect on automated or manual sequencing, restriction digestions or other downstream applications. However, if removing the dye is desired, this can easily be accomplished using any standard purification method.
No license is conveyed with the purchase of this product under any of US Patents Nos. 5,804,375, 5,994,056, 6,171,785, 6,214,979, 5,538,848, 5,723,591, 5,876,930, 6,030,787, and 6,258,569, and corresponding patents outside the United States, or any other patents or patent applications, relating to the 5′ Nuclease and dsDNA-Binding Dye Processes. For further information contact the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
REDTaq is a registered trademark of Sigma-Aldrich Co. LLC
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To be used with REDTaq® DNA Polymerase
Hot-start high fidelity Taq enzyme with inert dye, 10X buffer included
Complete PCR reagent with standard Taq DNA Polymerase and inert dye
with 10× PCR reaction buffer containing MgCl2, recombinant, expressed in E. coli
Certificate of Analysis
Innis, M.A., and Gelfand, D.H. PCR Protocols: A Guide to Methods and Applications, (1990), 3
Mapping networks of protein-mediated physical interactions between chromitin elements. Tiwari, V.K., and Baylin, S.B. Curr. Protoc. Mol. Biol., 21.1613, (2010)
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