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For routine PCR amplifications
• Same great performance as Taq DNA Polymerase in a more convenient format for high throughput applications.
• Visual confirmation that not only has the enzyme been added, but that proper mixing has occurred.
• No additional loading dyes are necessary. An aliquot can be taken directly from the reaction and loaded onto an agarose gel for electrophoresis.
Provided with 10X reaction buffer containing MgCl2
One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.
REDTaq DNA Polymerase is a unique blend of Sigma′s quality Taq DNA Polymerase combined with an inert red dye. This dye enables quick visual confirmation of enzyme addition and reaction mixing. An aliquot of the samples (5-10 μl) can then be loaded directly onto an agarose gel for electrophoresis following PCR. The red dye migrates slightly faster than bromophenol blue at about the same rate as a 125 base pair fragment in a 1% agarose gel. Since no additional loading buffers are added to the reaction following PCR, reamplification is possible.
The red dye has no effect on automated or manual sequencing, restriction digestions or other downstream applications. However, if removing the dye is desired, this can easily be accomplished using any standard purification method.
REDTaq is a registered trademark of Sigma-Aldrich Co. LLC
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
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To be used with REDTaq® DNA Polymerase
Hot-start Taq enzyme with inert dye, 10X buffer included
Complete PCR reagent with standard Taq DNA Polymerase and inert dye
with 10× PCR reaction buffer containing MgCl2
Certificate of Analysis
Since its introduction, REDTaq has been well received by the scientific community. The original product idea was to add convenience to PCR by formulating a reaction that also contained loading buffer...
by Brian Ward and Keming Song
Sigma-Aldrich Corporation, St. Louis, MO, USA
Keywords: Amplification, Error, Melting, Methods, Nucleic acid annealing, Peptide synthesis, Polymerase chain reaction, Purification, Sequencing, transformation
Taq DNA Polymerase is a thermostable enzyme derived from the thermophilic bacterium Thermus aquaticus. The enzyme is in a recombinant form, expressed in E. coli. It is able to withstand repeated heat...
Keywords: AGE, Amplification, Electrophoresis, Evaporation, Gel electrophoresis, PAGE, Polymerase chain reaction, Sequencing
Innis, M.A., and Gelfand, D.H. PCR Protocols: A Guide to Methods and Applications, (1990), 3
Mapping networks of protein-mediated physical interactions between chromitin elements. Tiwari, V.K., and Baylin, S.B. Curr. Protoc. Mol. Biol., 21.1613, (2010)
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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