• USA Home
  • D4513 - Deoxyribonuclease I from bovine pancreas

EMAIL THIS PAGE TO A FRIEND
D4513 Sigma

Deoxyribonuclease I from bovine pancreas

Type II-S, lyophilized powder, Protein ≥80 %, ≥2,000 units/mg protein

Synonym: DNase I, Deoxyribonucleate 5′-oligonucleotido-hydrolase

Purchase

Properties

Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Cell Biology,
Quality Level   PREMIUM
sterility   sterile-filtered
type   Type II-S
form   lyophilized powder
mol wt   mol wt ~31 kDa
purified by   chromatography
composition   Protein, ≥80%
packaging   vial of ≥10.0 mg protein
impurities   endotoxin, tested
solubility   0.15 M NaCl: soluble 5.0 mg/mL clear(lit.)
Featured Industry   Diagnostic Assay Manufacturing
Diagnostic Assay Manufacturing
foreign activity   Chymotrypsin ≤0.01%
  Protease ≤0.005%
  RNase ≤0.01%
storage temp.   −20°C

Description

Analysis Note

Protein determined by biuret.

Application

Used for the removal of DNA from protein samples.

DNAse I from Sigma has been compared with human urine-derived interleukin 1 inhibitor for the ability to hydrolyze [14C]DNA [14C]DNA. It has also been used to cleave a 139 base pair Hind III/Nci I restriction fragment to investigate the stability of the enzyme for use in footprinting experiments. DNase I is widely used as a footprinting agent for studying drug and protein binding to DNA.

Deoxyribonuclease I from bovine pancreas has been used in a study to investigate a new approach to obtaining high-activity RNase, DNase, cholesterolesterase, and trypsin from cattle pancreas. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the isolation and further characterization of carp liver DNase.

Preparation Note

The enzyme powder may be reconstituted in water or any buffer at pH 4.0-9.0, except phosphate buffer. Calcium chelators should be avoided. 10 mg/mL solution of DNAse I in 0.15 M NaCl may lose <10% of its activity when stored for a week in aliquots at −20 °C. The same solutions stored in aliquots at 2-8 °C can lose approximately 20% activity. It remains active for up to five hours at 60 °C between pH 5 and 7, and loses activity in <10 minutes at 68 °C. It loses activity at the rate of 6%/hour in acetate buffer (pH 5.0) and tris buffer ((pH 7.2) at 1 mg/mL concentration.

Unit Definition

One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 5.0 at 25 °C, using DNA, Type I or III as substrate.

Physical form

Lyophilized powder containing calcium chloride

Biochem/physiol Actions

DNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5′-phosphates. In the presence of Mg2+, DNAse I cleaves each strand of DNA independently and the cleavage sites are random. Both DNA strands are cleaved at approximately the same site in the presence of Mn2+. The pH optimum lies between 7 and 8. Divalent cations such as Mn2+, Ca2+, Co2+, and Zn2+ are activators of the enzyme. A concentration of 5 mM Ca2+ stabilizes the enzyme against proteolytic digestion. DNAse I from bovine pancreas consists of four chromatographically distinguishable components, A, B, C, and D, with their molar ratios being 4:1:1 respectively. Only minor amounts of D are found. 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS) and actin are known to inhibit the enzyme activity.

Price and Availability


All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
RF0750000
Protocols & Articles

Protocols

Enzymatic Assay of Deoxyribonuclease I (EC 3.1.21.1)

To standardize a procedure for the enzymatic assay of Deoxyribonuclease I at Sigma-Aldrich St. Louis.

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

Related Products

inhibitor

Product #

Description

Add to Cart

N9653 Netropsin dihydrochloride, from Streptomyces netropsis, ≥98% (HPLC and TLC), powder
D6518 Diethylenetriaminepentaacetic acid, ≥99% (titration)
P9375 1,10-Phenanthroline monohydrate, reagent grade
E4378 Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid, ≥97.0%
ED2SS Ethylenediaminetetraacetic acid disodium salt dihydrate, reagent grade, 98.5-101.5% (titration)

related product

Product #

Description

Add to Cart

92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?