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D4522 Sigma

Deoxyribonucleic acid from calf thymus

Type XV, Activated, lyophilized powder

Synonym: Calf Thymus DNA, DNA from calf thymus, Thymonucleic acid

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Properties

Related Categories DNA, Mammalian DNA, Molecular Biology, Molecular Biology Reagents
grade   for molecular biology
type   Type XV
form   lyophilized powder
storage temp.   −20°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard to determine DNA content in gels and for DNA quantification by fluorescent assay.

Features and Benefits

• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA

General description

High quality double-stranded template DNA isolated from the thymus of male and female calves.

Packaging

1, 5 mg in glass bottle

Preparation Note

Prepared from highly polymerized calf thymus DNA and nicked with DNase using the method of Aposhian and Kornberg.

Quantity

One mg DNA is approx. 20 A260 units.

Reconstitution

Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.

Price and Availability


Laboratory Gloves

Biomedical Applications
Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
Is Product D4522, Deoxyribonucleic acid, single or double stranded?
Even though it is activated or "nicked" DNA, we have found that it is primarily double stranded.
What is the solution stability of Product D4522, Deoxyribonucleic acid?
Solutions of deoxyribonucleic acid have been stored successfully for several months at 4°C, in 10 mM Tris, pH 7.5 - 8.0, with 1 mM EDTA and without a bacteriostatic agent.  At low concentrations (25 µg/mL), DNA tends to absorb onto the surfaces of plastic tubes.  It is not recommended to store deoxyribonucleic acid in alkaline solutions since it tends to degrade at pH greater than 8.0.  
What is the molecular weight of Product D4522, Deoxyribonucleic acid?
The molecular weight is reported to be between 10-15 million Daltons prior to activation (nicking).
Is BSA (bovine serum albumin) used during the method of preparation for Product D4522, Deoxyribonucleic acid?
During the method of preparation, when deoxyribonucleic acid is nicked, BSA is added to the solution.  This is followed by an extraction method to remove all exogenous protein. We do not, however, claim this deoxyribonucleic acid is 100% clean of all proteins (including BSA).
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
Show more questions
Protocols & Articles
Peer-Reviewed Papers
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References

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