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D4522 Sigma

Deoxyribonucleic acid from calf thymus

Type XV, Activated, lyophilized powder

Synonym: Calf Thymus DNA, DNA from calf thymus, Thymonucleic acid

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Properties

Related Categories DNA, Mammalian DNA, Molecular Biology, Molecular Biology Reagents
grade   for molecular biology
type   Type XV
form   lyophilized powder
storage temp.   −20°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard to determine DNA content in gels and for DNA quantification by fluorescent assay.

Features and Benefits

• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA

General description

High quality double-stranded template DNA isolated from the thymus of male and female calves.

Preparation Note

Prepared from highly polymerized calf thymus DNA and nicked with DNase using the method of Aposhian and Kornberg.

Quantity

One mg DNA is approx. 20 A260 units.

Reconstitution

Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.



Protocols & Articles
Peer-Reviewed Papers
15

References

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