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D4545 Sigma

Taq DNA Polymerase from Thermus aquaticus

with 10× PCR reaction buffer without MgCl2

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Properties

Related Categories 2.7.x.x Phosphorus containing groups, 2.x.x.x Transferases, Application Index, Biochemicals and Reagents, Core Bioreagents,
recombinant   expressed in E. coli
form   liquid
feature   hotstart: No
concentration   5 units/μL
color   colorless
  colorless
suitability   suitable for PCR and automated sequencing reactions
Featured Industry   Agriculture
shipped in   wet ice
storage temp.   −20°C

Description

Features and Benefits

• MgCl2 provided in a separate tube to allow MgCl2 optimization

Other Notes

Taq DNA Polymerase is a specialized thermostable enzyme isolated from the thermophilic bacterium Thermus aquaticus. The recombinant form of this enzyme is expressed in E. coli. This 94 kDa protein shows no detectable levels of contaminating endonucleases or exonucleases by SDS-PAGE. It has both 5′→3′ polymerase and exonuclease activity.

Packaging

Taq DNA Polymerase with 10× reaction buffer without MgCl2. Includes a separate tube of 25 mM MgCl2

Taq DNA polymerase comes with the choice of an optimized 10× reaction buffer including MgCl2 (D1806) or a 10× reaction buffer without MgCl2 plus a separate tube of MgCl2 for titration (D4545). The latter option may be necessary to determine optimal conditions for amplification.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.

Unit Definition

One unit will incorporate 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.

Application

Taq DNA Polymerase from Thermus aquaticus is a thermostable DNA polymerase that is used for the DNA polymerase chain reaction (PCR) in order to amplify DNA sequences.

Taq DNA Polymerase from Thermus aquaticus has been used in the process of DNA extraction (during gene amplification and sequencing). It has been used in genotyping. It has also been used in polymerase chain reaction (PCR) to study the constitutive production of epithelial neutrophil activating peptide 78 (ENA-78) and interleukin-8 (IL-8).

Biochem/physiol Actions

Taq polymerase catalyzes oligonucleotide primer-driven, DNA template dependent incorporation of dNTPs into complimentary DNA strands. It displays both 5′ to 3′ polymerase and exonuclease activities.

General description

Taq polymerase is a thermostable DNA polymerase named after the thermophilic bacterium Thermus aquaticus. A stable deoxyribonucleic acid (DNA) polymerase (with a temperature optimum of 80°C) has been purified from the extreme thermophile Thermus aquaticus. The enzyme is free from phosphomonoesterase, phosphodiesterase and single-stranded exonuclease activities. Maximal activity of the enzyme needs all four deoxyribonucleotides and activated calf thymus DNA.

Price and Availability


KiCqStart Probe Assays
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
Protocols & Articles

Articles

Improving Real-Time PCR Success

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The Polymerase Chain Reaction (PCR) is used in all areas of biological science research, including the clinical, forensic and diagnostic fields and the widespread adoption of the PCR technique has re...
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Polymerase Chain Reaction - PCR Technologies Guide

The polymerase chain reaction (PCR)1,2,3 has become one of the most widely used techniques in molecular biology. It is used in applications from basic research to high-throughput screening. While it ...
Keywords: Amplification, Cloning, Degradations, Detection methods, Electrophoresis, Evaporation, Gas chromatography, Gel electrophoresis, Indicators, Melting, Molecular biology, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Sequencing, Size-exclusion chromatography, Transcription

Protocols

Hot Start dNTP protocol to reduce non-specific amplification

· How do Hot-Start dNTPs work? · Handling · Protocols for Taq DNA Polymerase—Standard PCR, Fast PCR, Multiplexed PCR and Real-Time PCR · Troubleshooting · Standard Thermal Cycling Conditions for Othe...
Keywords: AGE, Amplification, Buffers, Centrifugation, Degradations, Electrophoresis, Gel electrophoresis, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Purification, Size-exclusion chromatography

Standard PCR protocol

Taq DNA Polymerase is a thermostable enzyme derived from the thermophilic bacterium Thermus aquaticus. The enzyme is in a recombinant form, expressed in E. coli. It is able to withstand repeated heat...
Keywords: AGE, Amplification, Applications, Electrophoresis, Evaporation, Gel electrophoresis, PAGE, Polymerase chain reaction, Sequencing

dNTP Mediated Hot Start PCR Protocol

Hot Start dNTPs are modified with a thermolabile protecting group at the 3’ terminus. The presence of this modification blocks nucleotide incorporation by DNA polymerase until the nucleotide protecti...
Keywords: AGE, Amplification, Buffers, Centrifugation, Degradations, Electrophoresis, Gel electrophoresis, Nucleic acid denaturation, Polymerase chain reaction, Purification, Size-exclusion chromatography, Transcription

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PCR Selection Guide

Sigma-Aldrich offers a wide variety of PCR reagents to meet any experimental needs. Our range of polymerases is customized to meet your End-Point PCR, qPCR, or RT-PCR needs. Our products vary from ro...
Keywords: Polymerase chain reaction, Polymerase chain reaction - quantitative

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Peer-Reviewed Papers
15

References

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