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D4551 Sigma

N,N-Dimethylformamide

for molecular biology, ≥99%

Synonym: DMF, NSC 5356

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Properties

Related Categories Biochemicals, Core Bioreagents, Dimethylformamide (DMF), Life Science Reagents for Protein Expression and Purification, Life Science Reagents for Transfection,
grade   for molecular biology
vapor density   2.5 (vs air)
vapor pressure   2.7 mmHg ( 20 °C)
InChI Key   ZMXDDKWLCZADIW-UHFFFAOYSA-N
assay   ≥99%
form   liquid
autoignition temp.   833 °F
expl. lim.   15.2 %
refractive index   n20/D 1.430(lit.)
bp   153 °C(lit.)
mp   −61 °C(lit.)
density   0.944 g/mL(lit.)
suitability   suitable for solvent for chromogenic substrates (X-Gal, etc.) used in molecular biology applications.
storage temp.   room temp

Description

Application

Solvent for many hydrophobic organic compounds.

N,N-Dimethylformamide was used to solubilize MTT crystals in cell viability assays. It was also used in feruloyl esterase activity assay in molds exhibiting high activity of the enzyme.

General description

N, N-Dimethylformamide (DMF) is a solvent with a low evaporation rate, useful for preparing solutions with a variety of hydrophobic organic compounds used in molecular biology applications.

Packaging

250, 500 mL in glass bottle

Price and Availability

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 2265 3 / PGIII
WGK Germany 
2
RTECS 
LQ2100000
Flash Point(F) 
136.4 °F
Flash Point(C) 
58 °C
Protocols & Articles

Articles

N,N-Dimethylformamide Molecular Biology Reagent

DMF is a polar organic solvent that is widely used in the synthesis of organic compounds. The high solvent power and stability of DMF at elevated temperatures contribute to its utility. DMF is a solv...
Keywords: Affinity chromatography, Capillary electrophoresis, Carboxylations, Chromatography, Crystallization, Electrophoresis, High performance liquid chromatography, Melting, Molecular biology, Separation, Solvents

Recombinant Protein Expression in Saccharomyces cerevisiae

Expressing recombinant proteins in yeast has many advantages, simple protocols, use of basic reagents, appropriate processing of eukaryotic proteins, and scalability. Sigma® Life Science is an essent...
Keywords: Cell disruption, Gene expression, transformation

Protocols

Selecting Correctly Expressing Recombinants

Blue / White colony screening is a strategy to quickly and easily distinguish between recombinant and non-recombinant colonies. It requires a special vector and a special strain of E. coli. It is par...
Keywords: AGE, Amplification, Antibiotics, Cell disruption, Centrifugation, Cloning, Degradations, Detergents, Diagnostic, Digestions, Electrophoresis, Gel electrophoresis, Molecular biology, Peptide synthesis, Phase transitions, Polymerase chain reaction, Precipitation, Purification, Sequencing, Transfection, transformation

Peer-Reviewed Papers
15

References

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