|publication info||K.I. Mills and B.H. Ramsahoye, ed., Humana Press, 2002, 192 pp., hard cover|
This book offers a set of readily reproducible protocols of the analysis of DNA methylation and methylases. These powerful methods provide the tools necessary for studying methylation at both the global level and the level of sequence, and include many techniques for identifying genes that might be aberrantly methylated in cancer and aging. Additional methods cover genome-wide analysis of abnormal DNA methylation and the isolation and measurement of demethylases and related proteins.
Measurement of Genome-Wide DNA Cytosine-5 Methylation by Reversed-Phase High-Pressure Liquid Chromatography
Methylation Analysis by Chemical DNA Sequencing
Methylation-Sensitive Restriction Fingerprinting
Restriction Landmark Genome Scanning
Combined Bisulfite Restriction Analysis (COBRA)
Differential Methylation Hybridization Using CpG Island Arrays
Methylated CpG Island Amplification for Methylation Analysis and Cloning Differentially Methylated Sequences
Isolation of CpG Islands Using a Methyl-CpG Binding Column
Purification of MeCP2-Containing Deacetylase from Xenopus laevis
DNA-Methylation Analysis by the Bisulfite-Assisted Genomic Sequencing Method
Measuring DNA Demethylase Activity In Vitro
Extracting DNA Demethylase Activity from Mammalian Cells
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sufficient for 10 purifications
Complete ChIP reaction in 6 hours in flexible strip well format
For bisulfite DNA conversion & purification
To measure global DNA methylation shifts from as low as 10 ng DNA
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