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D4764 Sigma

Deoxyribonucleic acid from calf thymus

Genomic, unsheared

Synonym: Calf Thymus DNA, DNA from calf thymus, Thymonucleic acid

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Properties

Related Categories DNA, Genomic DNA, Molecular Biology, Molecular Biology Reagents
grade   for molecular biology
form   lyophilized powder
mol wt   mol wt 8.0-15 kb
storage temp.   2-8°C

Description

Application

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard for DNA quantification by fluorescent assay. It was used as reference to determine the DNA content in cartilage tissue digest samples.

Features and Benefits

• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA

General description

High quality double-stranded template DNA isolated from the thymus of male and female calves.

Packaging

0.25, 1 unit in glass bottle

5 units in glass bottle

Preparation Note

Purified by equilibrium buoyant density ultracentrifugation in cesium chloride, dialyzed against a solution of 1 mM sodium chloride, 1 mM EDTA and 1 mM Trizma HCl, pH 7.5 and lyophilized at a concentration of ~2 A260 units per mL. ~20 A260 units per mg DNA.

Reconstitution

Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.

Specificity

42% GC; Tm = 87 °C.
% GC is the percentage of G/C base pairs. Tm is the temperature at the midpoint of the thermal denaturation profile.

Price and Availability


Laboratory Gloves

Biomedical Applications
Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles
Peer-Reviewed Papers
15

References

Related Products

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Product #

Description

Add to Cart

D8899 Deoxyribonucleic acid, single stranded from calf thymus, lyophilized powder
GE27-4575-01 DNA (Calf Thymus), activated, GE Healthcare, 27-4575-01, pack of 1 ea
08168 Timestrip Plus 8 °C
D4416 Deoxyribonucleic acid, High molecular weight from BALB/c mouse, adult, solution

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