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D4812 Sigma

REDAccuTaq® LA DNA Polymerase

High fidelity Taq with inert dye, 10X buffer included

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Properties

Related Categories Long & Accurate PCR, Molecular Biology, PCR/Amplification More...
form   liquid
feature   hotstart: No
concentration   1 unit/μL
color   red
  red
shipped in   wet ice
storage temp.   −20°C

Description

Features and Benefits

• Increased fidelity, up to 6.5× that of Taq DNA polymerase
• Efficiently and accurately produce amplicons up to 22 kb on genomic templates and up to 40 kb on less complex templates such as lambda or bacterial DNA
• Visual confirmation that enzyme has been added, and proper mixing has occurred
• No additional loading dyes needed. A post-reaction aliquot can be directly used on an agarose gel for electrophoresis

General description

REDAccuTaq LA DNA polymerase allows for quick recognition in high throughput applications as well as direct loading of amplification products onto agarose gels for electrophoresis. The inert red dye has no effect on automated sequencing, restriction enzyme digestion, ligation, or other downstream manipulations. However, the PCR product is easily separated from the dye by standard purification methods.

Other Notes

REDAccuTaq® LA DNA polymerase is a blend of Sigma′s high quality Taq DNA polymerase, a small amount of an additional polymerase that exhibits 3′→5′ exonuclease or proofreading activity, and our inert red dye. By blending the Taq with the right amount of this proofreading enzyme, misincorporation errors are corrected, producing PCR amplicons that are longer and more accurate.

Store REDAccuTaq LA DNA Polymerase and AccuTaq LA 10x Buffer at –20 °C.

Packaging

The enzyme is provided with an optimized 10× reaction buffer for enhanced amplification of complex templates.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

REDAccuTaq is a registered trademark of Sigma-Aldrich Co. LLC

Unit Definition

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C.

Application

Produce long DNA amplicons for:
• Mutation analysis
• Cloning genes
• DNA sequencing
• cDNA library generation

Price and Availability


Ultrapure dNTPs for PCR

Kapa Biosystems
Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Protocols

Applications with REDTaq

Since its introduction, REDTaq has been well received by the scientific community. The original product idea was to add convenience to PCR by formulating a reaction that also contained loading buffer...
by Brian Ward and Keming Song
Sigma-Aldrich Corporation, St. Louis, MO, USA
Keywords: Amplification, Applications, Error, Melting, Methods, Nucleic acid annealing, Peptide synthesis, Polymerase chain reaction, Purification, Sequencing, transformation

End Point PCR Protocol for Long and Accurate DNA Amplification

Technology Overview Equipment Reagents Assay Considerations Procedure Troubleshooting Materials References

Long and Accurate PCR Amplification of DNA with RedAccuTaq D4812 Protocol

Reaction Optimization Reliable amplification of long DNA sequences requires: 1) effective denaturation of DNA template, 2) adequate extension times to produce large products and 3) protection of targ...

Related Content

PCR Selection Guide

Sigma-Aldrich offers a wide variety of PCR reagents to meet any experimental needs. Our range of polymerases is customized to meet your End-Point PCR, qPCR, or RT-PCR needs. Our products vary from ro...
Keywords: Polymerase chain reaction, Polymerase chain reaction - quantitative

Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
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D8045 AccuTaq LA DNA Polymerase, High fidelity Taq enzyme, with 10X buffer & DMSO
D1313 JumpStart REDAccuTaq® LA DNA Polymerase, Long and accurate hot-start Taq with inert dye, 10X buffer included
D5809 JumpStart AccuTaq LA DNA Polymerase, Hot-start high fidelity Taq enzyme, 10X buffer included
92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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