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D4820 Sigma

Anti-DNASE1 (91-105) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-Deoxyribonuclease-1 precursor, Anti-DNL1, Anti-DRNI

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Properties

Related Categories Alphabetical Index, Antibodies, DM-DP, Primary Antibodies
species reactivity   human
application(s)   western blot: 1:250
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt ~31 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... DNASE1(1773)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 91-105 of human DNASE1

General description

The enzyme is Ca2+ and Mg2+ dependent. It is synthesized in the pancreas and stored in zymogen granules. It has been used to reduce the viscosity of cystic fibrosis sputum.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Anti-DNASE1 antibody produced in rabbit is suitable for western blot analysis at a working dilution of 1:250. Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about the Sigma Life Science and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.

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Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Biochem/physiol Actions

DNase I is an endonuclease that hydrolyzes double-stranded or single-stranded DNA preferentially at sites adjacent to pyrimidine nucleotides to produce a complex mixture of 5′-phosphate mononucleotides and oligonucleotides. Mutations in this gene is associated with systemic lupus erythematosus (SLE), an autoimmune disease.

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Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

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