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D4902 Sigma

Dexamethasone

powder, BioReagent, suitable for cell culture, ≥97%

Synonym: (11β,16α)-9-Fluoro-11,17,21-trihydroxy-16-methylpregna-1,4-diene-3,20-dione, 9α-Fluoro-16α-methyl-11β,17α,21-trihydroxy-1,4-pregnadiene-3,20-dione, 9α-Fluoro-16α-methylprednisolone, Prednisolone F

  • CAS Number 50-02-2

  • Empirical Formula (Hill Notation) C22H29FO5

  • Molecular Weight 392.46

  •  Beilstein Registry Number 2066651

  •  EC Number 200-003-9

  •  MDL number MFCD00064136

  •  PubChem Substance ID 24893906

  • Popular Documents:  FTNMR (PDF)  

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Properties

Related Categories Bioactives and Supplements for Stem Cell Expansion, Bioactives/Supplements, Cell Biology, Cell Culture, Cell Signaling and Neuroscience,
InChI Key   UREBDLICKHMUKA-CXSFZGCWSA-N
product line   BioReagent
assay   ≥97%
form   powder
potency   4-500 ng/mL
mp   262-264 °C(lit.)
solubility   methanol: soluble25 mg/mL
suitability   suitable for cell culture
storage temp.   2-8°C
Gene Information   human ... ABCB1(5243), CYP3A4(1576), IL4(3565), IL5(3567), NR3C1(2908)
mouse ... Abcb1a(18671), Abcb1b(18669), Ifng(15978), Nos2(18126), Ptgs2(19225), Tnf(21926)
rat ... Ar(24208), Nr3c1(24413), Tnf(24835)

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Packaging

1 g in glass bottle

25, 100, 500 mg in glass bottle

Bottomless glass bottle. Contents are inside inserted fused cone.

Reconstitution

To prepare 20 μg/ml stock solution, add 1ml absolute ethanol per mg product; gently swirl to dissolve. Add 49 ml sterile medium per ml of ethanol added, while mixing, to acheive final concentration of 20 μg/ml.

Physical form

powder 2-8 °C; stock-frozen in working aliquots, avoid repeated freeze/thaw

Application

Dexamethasone is used to study apoptosis, cell signaling pathways, cell differentiation and gene expression.

Biochem/physiol Actions

Glucocorticoid anti-inflammatory agent. Regulates T cell survival, growth, and differentiation. Inhibits the induction of nitric oxide synthase.

It is poorly metabolized in the body and has great affinity and bioavailability.

Price and Availability


Filter out uncertainty.

All labs need water
Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
2
RTECS 
TU3980000

Frequently Asked Questions

What is Product D4902, Dexamethasone, soluble in?
Dexamethasone is practically insoluble in water. It is soluble in ethanol, methanol, acetone, dioxane and slightly soluble in chloroform. 
How should I reconstitute Product D4902, Dexamethasone, for tissue culture use?
The tissue culture grade product is dissolved in ethanol at a concentration of 1 mg/ml forming a clear, colorless solution.  It is also possible to prepare a stock solution of dexamethasone in DMSO at 1.5 mM.  
Do you have references for the use of Product D4902, Dexamethasone?
References can be found on the product detail page:Migliorati, G., et al., Dexamethasone and interleukins modulate apoptosis of murine thymocytes and peripheral T-lymphocytes. Pharmacol Res., 30(1), 43-52 (1994).Bansal, N., et al., Apoptosis: mode of cell death induced in T cell leukemia lines by dexamethasone and other agents. FASEB J. 5(2), 211-6 (1991).Shiao, R.T., et al., Dexamethasone and suramin inhibit cell proliferation and interleukin-6-mediated immunoglobulin secretion in human lymphoid and multiple myeloma cell lines. Leuk. Lymphoma, 17(5-6), 485-494 (1995).Migliorati, G., et al, Dexamethasone induces apoptosis in mouse natural killer cells and cytotoxic T lymphocytes. Immunology, 81(1), 21-26 (1994). Click on the following link to view the list of references on the product detail page:http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/D4902
How long is Product D4902, Dexamethasone, stable in solution?
Dexamethasone solutions are stable for at least 30 days when stored at 4 °C and protected from light. The solution may be aliquoted and stored at -20 °C. However, insufficient information is available to assess the shelf-life of the frozen solution.
What cell lines has Product D4902, Dexamethasone, been tested on?
The cell lines on which this product is tested can vary from lot to lot. Some typically used cell lines are 3T3 fibroblasts, BHK, L929 and CHO.
What is the shelf life of Product D4902, Dexamethasone?
The product has a 5 year shelf life. Please click on the following link to view the specification sheet for this product:  http://www.sigmaaldrich.com/catalog/DataSheetPage.do?brandKey=SIGMA&symbol=D4902
Product D4902, Dexamethasone is not water soluble. Do you have a water soluble Dexamethasone?
Product D2915 is water soluble. To find more information on D2915 click on the following link:  http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/D2915
Can I use Product D4902, Dexamethasone, to inject into mice?
We do not perform animal studies with our products, but it can be used in cell culture (D2915 - cell culture grade version, or USP grade product D9184). Please click the following links to view product information on these two products:  http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/D2915 http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/D9184
Are Products D4902 and D2915, sterile?
No. This product is not sterile, but cell culture testing did not find the product to be toxic to cells.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles

Articles

Mitochondrial Stress and ROS

Oxidative stress is implicated in the pathogenesis of lipotoxicity in both animal and human studies. Chronic oxidative stress is linked to insulin resistance in multiple tissues. Oxidative stress is ...
BioFiles 2011, 6.4, 22.
Keywords: Anti-inflammatory agents, Apoptosis, Cancer, Cell signaling, Cellular processes, Gene expression, Indicators, Metabolism, Oxidations, Respiratory

Peer-Reviewed Papers
15

References

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