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D4904 Sigma

pBR322 Plasmid DNA from E. coli RRI

buffered aqueous solution

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Properties

grade   for molecular biology
form   buffered aqueous solution
mol wt   mol wt 2.9 MDa
  mol wt 4363 bp
shipped in   dry ice
storage temp.   −20°C

Description

Components

DNA is provided in a solution of 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.

Application

Suitable for use in:
• Cloning
• Selection and expression of recombinant molecules
• Construction of shuttle vectors
• Construction of vectors for nucleotide sequencing
• Studies of elements involved in gene expression
• As plasmid DNA standards
• As a model system for studies on prokaryotic plasmid replication

Plasmid pBR322 was one of the first multipurpose cloning vectors constructed for use in Escherichia coli. This plasmid and derivatives have been used for a number of purposes including cloning, selection and expression of recombinant molecules, construction of shuttle vectors and vectors for nucleotide sequencing, studies of elements involved in gene expression, as plasmid DNA standards, and as a model system for studies on prokaryotic plasmid replication.

General description

Plasmid pBR322 was one of the first multipurpose cloning vectors constructed for use in E. coli. This plasmid is derived from the ColE1-type plasmid pMB1 and shares the same type of replication mechanism and controls as ColE1 and relatives. Plasmid pBR322 confers resistance to ampicillin and tetracycline. The plasmid sequence has been published.

The plasmid has unique restriction sites within the gene for ampicillin resistance (Pst I, Pvu I, and Sca I), within the gene for tetracycline resistance (BamH I, BspM I, EcoR V, Nhe I, Nru I, Sal I, Sph I, and Xma III), and elsewhere (Aat II, Ava I, Bal I, Bsm I, BspM II, Cla I, EcoR I, Hind III, Nde I, Pvu II, Ssp I, Sty I, and Tth111 I).

Other Notes

Accession number: J01749

Packaging

0.25, 1 unit in poly bottle

5 units in poly bottle

Specificity

Unique Sites: Within the gene for ampicillin resistance: Pst I, Pvu I, Sca I; Within the gene for tetracycline resistance: BamH I, BspM I, EcoR V, Nhe I, Nru I, Sal I, Sph I, Xma III; other unique sites: Aat II, Ava I, Bal I, Bsm I, BspM II, Cla I, EcoR I, Hind III, Nde I, Pvu II, Ssp I, Sty I, Tth111 I.

Biochem/physiol Actions

This plasmid is derived from the ColE1-type plasmid pMB1 and shares the same type of replication mechanism and controls as ColE1 and relatives.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles
Peer-Reviewed Papers
15

References

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