Synonym: DNase I, Deoxyribonucleate 5′-oligonucleotido-hydrolase
|Related Categories||3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Apoptosis and Cell Cycle, Application Index, Biochemicals and Reagents,|
|mol wt||mol wt ~31 kDa|
|foreign activity||Chymotrypsin ≤0.5%|
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Protein determined by biuret.
Used for the removal of DNA from protein samples.
Deoxyribonuclease I from bovine pancreas has been used in a study to investigate a two-dimensional zymogram analysis of nucleases in Bacillus subtilis. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effects of minor and major groove-binding drugs and intercalators on the DNA association of minor groove-binding proteins RecA and deoxyribonuclease I.
Derived from New Zealand-sourced pancreas
One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 5.0 at 25 °C, using DNA, Type I or III as substrate. [Mg2+] = 4.2 mM.
Lyophilized powder containing calcium chloride
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1. OBJECTIVE To standardize a procedure for the enzymatic assay of Deoxyribonuclease I at Sigma-Aldrich St. Louis.
The structure of PstI-repeats adjacent to the growth hormone gene and transcription of the family of PstI-repeats in various bovine organs Akopian, K., et al. Mol. Biol. 24, 775-80, (1990)
Effects of minor and major groove-binding drugs and intercalators on the DNA association of minor groove-binding proteins RecA and deoxyribonuclease I detected by flow linear dichroism Tuite, E., et al. Eur. J. Biochem. 243, 482-92, (1997)
Laskowski, M. The Enzymes 3rd ed.,, (1971) 4, 289
Moore, S. The Enzymes 3rd ed.,, (1981) 14, 281
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