Deoxyribonuclease I from bovine pancreas
Type IV, lyophilized powder, ≥2,000 Kunitz units/mg protein
DOWNLOAD MSDS (PDF)Synonym: DNase I, Deoxyribonucleate 5′-oligonucleotido-hydrolase
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CAS Number 9003-98-9
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EC Number 232-667-0
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MDL number MFCD00130918
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Popular Documents: Datasheet (PDF) | Specification Sheet (PDF)
Properties
| Related Categories | 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Apoptosis and Cell Cycle, Application Index, Biochemicals and Reagents, |
| Quality Level | PREMIUM |
| type | Type IV |
| form | lyophilized powder |
| mol wt | mol wt ~31 kDa |
| purified by | chromatography |
| foreign activity | Chymotrypsin ≤0.5% |
| Protease ≤0.05% |
Description
Frequently Asked Questions
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Analysis Note
Protein determined by biuret.
Application
Used for the removal of DNA from protein samples.
Deoxyribonuclease I from bovine pancreas has been used in a study to investigate a two-dimensional zymogram analysis of nucleases in Bacillus subtilis. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effects of minor and major groove-binding drugs and intercalators on the DNA association of minor groove-binding proteins RecA and deoxyribonuclease I.
Preparation Note
Derived from New Zealand-sourced pancreas
Unit Definition
One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 5.0 at 25 °C, using DNA, Type I or III as substrate. [Mg2+] = 4.2 mM.
Physical form
Lyophilized powder containing calcium chloride
Price and Availability
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