Synonym: DNase I, Deoxyribonucleate 5′-oligonucleotido-hydrolase
|Related Categories||3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Apoptosis and Cell Cycle, Application Index, Biochemicals and Reagents,|
|mol wt||mol wt ~31 kDa|
|foreign activity||Chymotrypsin ≤0.5%|
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Protein determined by biuret.
Used for the removal of DNA from protein samples.
Deoxyribonuclease I from bovine pancreas has been used in a study to investigate a two-dimensional zymogram analysis of nucleases in Bacillus subtilis. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effects of minor and major groove-binding drugs and intercalators on the DNA association of minor groove-binding proteins RecA and deoxyribonuclease I.
Derived from New Zealand-sourced pancreas
One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 5.0 at 25 °C, using DNA, Type I or III as substrate. [Mg2+] = 4.2 mM.
Lyophilized powder containing calcium chloride
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Standardized vial containing 2,000 Kunitz units of DNase I (D4527), vial of ≥0.5 mg total protein
lyophilized powder, Protein ≥85 %, ≥400 Kunitz units/mg protein
1. OBJECTIVE To standardize a procedure for the enzymatic assay of Deoxyribonuclease I at Sigma-Aldrich St. Louis.
From our library of Related Content, Sigma-Aldrich presents Enzyme Explorer: the most comprehensive source of enzymes, substrates, activators, & inhibitors.
Keywords: Cell culture, Cell disruption, Cell signaling, Diagnostic, Digestions, Drug discovery, Functional genomics, Gene expression, Genomics, Metabolic Pathways, Molecular biology, Neuroscience, Proteomics
The structure of PstI-repeats adjacent to the growth hormone gene and transcription of the family of PstI-repeats in various bovine organs Akopian, K., et al. Mol. Biol. 24, 775-80, (1990)
Effects of minor and major groove-binding drugs and intercalators on the DNA association of minor groove-binding proteins RecA and deoxyribonuclease I detected by flow linear dichroism Tuite, E., et al. Eur. J. Biochem. 243, 482-92, (1997)
Ocular surface extracellular DNA and nuclease activity imbalance: a new paradigm for inflammation in dry eye disease. Sonawane S, Khanolkar V, Namavari A, et al. Invest. Ophthalmol. Vis. Sci. 53(13), 8253-63, (2012)
The structure of human DNase I bound to magnesium and phosphate ions points to a catalytic mechanism common to members of the DNase I-like superfamily. Parsiegla G, Noguere C, Santell L, et al. Biochemistry 51(51), 10250-8, (2012)
Identification of a target gene and activating stimulus for the YpdA/YpdB histidine kinase/response regulator system in Escherichia coli. Fried L, Behr S, and Jung K J. Bacteriol. 195(4), 807-15, (2013)
Methylome of DNase I sensitive chromatin in Populus trichocarpa shoot apical meristematic cells: a simplified approach revealing characteristics of gene-body DNA methylation in open chromatin state. Lafon-Placette C, Faivre-Rampant P, Delaunay A, et al. New Phytol. 197(2), 416-30, (2013)
Vκ gene repertoire and locus contraction are specified by critical DNase I hypersensitive sites within the Vκ-Jκ intervening region. Xiang Y, Park SK, and Garrard WT J. Immunol. 190(4), 1819-26, (2013)
Involvement of cyclic AMP receptor protein in regulation of the rmf gene encoding the ribosome modulation factor in Escherichia coli. Shimada T, Yoshida H, and Ishihama A J. Bacteriol. 195(10), 2212-9, (2013)
Laskowski, M. The Enzymes 3rd ed.,, (1971) 4, 289
Moore, S. The Enzymes 3rd ed.,, (1981) 14, 281
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