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D5030 Sigma

Dulbecco′s Modified Eagle′s Medium

Without glucose, L-glutamine, phenol red, sodium pyruvate and sodium bicarbonate, powder, suitable for cell culture

Synonym: DME, DMEM

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Properties

Related Categories Cell Biology, Cell Culture, Classic Media and Salts, Classical Media, Culture,
Quality Level   GMP
form   powder
suitability   suitable for cell culture
components   L-glutamine: no
NaHCO3: no
HEPES: no
sodium pyruvate: no
glucose: no
phenol red: no
storage temp.   2-8°C

Description

General description

The most basic formulation offered. This formulation is used by investigators who want to start with the essential components of DME, and have the flexibility to optimize the formula for their own application.

Quantity

Formulated to contain 8.3 grams of powder per liter of medium.

Reconstitution

Supplement with 1.0 g/L glucose, 0.584 g/L L-glutamine, 3.7 g/L sodium bicarbonate.

Protocols & Applications

Cryostor Cryopreservation Video Protocol

Price and Availability


All labs need water

Stericup and Steritop Filter Units
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Protocols

Cultrex® 3-D Culture Matrix™ Basement Membrane Extract Coated 96 Well Plate Protocol

3-D Culture is an innovative approach to modeling the morphological effects of oncogenesis and development using three-dimensional microenvironments. Extracellular matrix proteins form hydrogels unde...
Keywords: Cell culture, Diagnostic, Growth factors, Polymerase chain reaction

Cultrex® 3-D Culture Matrix™ Laminin I Protocol

3D Culture is a cell culture method that provides cells with the necessary structure and signaling cues to direct reconstruction of the tissue architecture, and these methods provide physiologically ...
Keywords: Cancer, Cell attachment, Cell culture, Diagnostic, Growth factors, Molecular probes, PAGE, Polymerase chain reaction

Cultrex® 3-D Culture Matrix™ Reduced Growth Factor Basement Membrane Extract, PathClear® Protocol

3D Culture is a cell culture method that provides cells with the necessary structure and signaling cues to direct reconstruction of the tissue architecture, and these methods provide physiologically ...
Keywords: Cancer, Cell culture, Diagnostic, Growth factors, Molecular probes, Polymerase chain reaction

Cultrex® Basement Membrane Extract, Type 2, PathClear® Protocol

Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma. Basement memb...
Keywords: Adhesion, Angiogenesis, Cell attachment, Cell culture, Diagnostic, Polymerase chain reaction

Cultrex® Reduced Growth Factor Basement Membrane Extract, Type 2, PathClear® Protocol

Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma. Basement memb...
Keywords: Adhesion, Angiogenesis, Cell attachment, Cell culture, Diagnostic, Polymerase chain reaction

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture. The entire contents of each package should be used immediately after opening. Preparing m...

Related Content

3D培养基底膜抽提物

3D细胞培养可为细胞提供必要的结构和信号指令来定向重建组织结构。这一类方法能够提供体外的生理性预期模型,帮助用户评估细胞的发育与疾病状态。正常细胞会聚集为结构类似其来源组织的类器官,进而表现出极化的形态,细胞周期调控现象并表达组织特异性的蛋白1-6。当癌症细胞聚集为肿瘤样结构时,则会基于其恶性程度,表现出组织性结构或细胞周期调控的缺乏,并表达出肿瘤特异性的标记物7-9。
Keywords: Cancer, Gene expression, Growth factors, Molecular probes, Morphogenesis

Dulbecco's Modified Eagle's Medium - DME, DMEM

Many modifications of Eagle's Medium have been developed since the original formulation appeared in the literature. Among the most widely used of these modifications is Dulbecco's Modified Eagle's me...
Keywords: Cell culture, Substitutions, Vitamins

Peer-Reviewed Papers
15

References

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G7513 L-Glutamine solution, 200 mM, solution, sterile-filtered, BioXtra, suitable for cell culture
P3532 Phenol Red, powder, BioReagent, suitable for cell culture
S5761 Sodium bicarbonate, powder, BioReagent, for molecular biology, suitable for cell culture, suitable for insect cell culture

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