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D5042 Sigma

123 bp DNA Ladder

for DNA electrophoresis

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Properties

Related Categories Core Bioreagents, DNA Ladder Markers, Life Science Reagents for DNA/RNA Electrophoresis, Markers and Ladders, Molecular Biology,
form   liquid
usage   100 uses (0.1 mg size)
  250 uses (250 μg size)
suitability   suitable for electrophoresis (DNA)
storage temp.   −20°C

Description

Components

Sigma’s 123 bp DNA Ladder is provided in a solution of 10 mM Tris-HCl (pH 7.5), 50 mM NaCl, and 0.1 mM EDTA.

General description

Sigma’s 123 bp DNA Step Ladder contains 35 dsDNA fragments from 123 to 4,182 bp. The fragments are 1 - 34 repeats of a 123 bp region of the rat prolactin gene. Ten ul of the marker should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Other Notes

Contains 35 fragments, 123 - 4182 bp

For optimal resolution, the recommended agarose gel concentration is 1.5%.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Introduction to Markers and Ladders

Sigma-Aldrich offers a variety of markers that aid size determination of samples separated by agarose and/or polyacrylamide gel electrophoresis. These products include markers for DNA, PCR fragments ...
Keywords: Digestions, Electrophoresis, Gel electrophoresis, PAGE, Polymerase chain reaction

Markers and Ladders Selection Guide

DNA and RNA size markers contain a mixture of DNA (or RNA) fragments of known length, making them suitable for estimating the fragment length of concurrently run samples. They stain well with ethidiu...
Keywords: AGE, Electrophoresis, Gel electrophoresis, PAGE, Polymerase chain reaction

Peer-Reviewed Papers
15

References

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D3937 DirectLoad 1 kb DNA Ladder, ready-to-use marker for DNA electrophoresis
D0428 1 kb DNA Ladder, for DNA electrophoresis
S7025 50 bp DNA Step Ladder, for DNA electrophoresis

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