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  • D5167 - Monoclonal Anti-Dynein (Intermediate Chain) antibody produced in mouse

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D5167 Sigma

Monoclonal Anti-Dynein (Intermediate Chain) antibody produced in mouse

clone 70.1, ascites fluid

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Cytoskeleton,
species reactivity   chicken, marsupial, frog, human, rat, hamster, Indian muntjac (Muntiacus muntjac vaginalis)
application(s)   immunoprecipitation: suitable
  indirect ELISA: suitable
  western blot: 1:2,000 using a chick brain extract
clone   70.1, monoclonal
antibody form   ascites fluid
isotype   IgM
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... DNAI1(27019), DNAI2(64446)
rat ... Dnai2(360654)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

cytoplasmic dynein from chicken embryo brain

General description

Dynein is a multimeric motor protein which transports membrane-bound vesicles, proteins and organelles towards the negative end of microtubules (retrograde transport). It has a crucial role in cytoplasmic motility, chromosomal movement and the organization of the Golgi apparatus. Monoclonal anti-dynein (Intermediate Chain) antibody can be used for localization of dynein intermediate chain using different immunochemical assays like ELISA, immunoblotting and dot blot. Mouse anti- dynein (Intermediate Chain) antibody reacts specifically with dynein of frog, hamster (CHO cell line), chicken, rat, marsupial (rat kangaroo, PtK1 cell line), Indian muntjac (Muntiacus muntjac vaginalis) and human (HeLa,MCF-7).

Application

Monoclonal anti-dynein (Intermediate Chain) antibody (diluted 1:500) can be used in western blotting and immunofluorescence microscopy. It can also be used in immunocytochemistry and dynein immunopurification.

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  2
Protocols & Articles

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
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References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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