Properties
| Related Categories | DNA Digest Markers, Molecular Biology, Nucleic Acid Electrophoresis, Nucleic Acid Markers |
| form | buffered aqueous solution |
| shipped in | dry ice |
| storage temp. | −20°C |
Description
Other Notes
Contains 6 fragments, 3,530–21,226 bp
Physical form
Solution in 10 mM Tris-HCl, pH 8.0, 1 mM EDTA
Application
EcoR I digested bacteria phage lambda DNA is suitable for size determination of double-stranded DNA using DNA electrophoresis. The recommended agarose gel concentration is 0.7% for this marker. A sample of the marker should be diluted with gel loading buffer to an appropriate loading concentration. Typically 1 μg per well (0.1 μg/μl, 10 μl load) is sufficient to be seen using ethidium bromide staining.
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