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D5523 Sigma

Dulbecco’s Modified Eagle’s Medium - low glucose

With 1000 mg/L glucose and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonym: DME, DMEM

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Properties

Related Categories Cell Biology, Cell Culture, Classic Media and Salts, Classical Media, Culture,
Quality Level   GMP
form   powder
suitability   suitable for cell culture
components   phenol red: yes
HEPES: no
glucose: low
NaHCO3: no
sodium pyruvate: yes
L-glutamine: yes
storage temp.   2-8°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Provides investigator with the option to modify the buffering system.

Quantity

Formulated to contain 10.0 grams of powder per liter of medium.

Reconstitution

Supplement with 3.7 g/L sodium bicarbonate.

Protocols & Applications

Mycoplasma PCR Detection Kit Video Protocol

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
What is the formulation of Product D5523, Dulbecco’s Modified Eagle’s Medium - low glucose?
The formulation can be found on the DMEM formulation sheet at the following link.
How do I prepare Product D5523, Dulbecco’s Modified Eagle’s Medium - low glucose?
Measure out 90% of final required volume of water. Water temperature should be 15-20 °C. While gently stirring the water, add the powdered medium. Stir until dissolved. Do NOT heat. Rinse original package with a small amount of water to remove all traces of powder. Add to solution in step 2. To the solution in step 3, add 3.7 g sodium bicarbonate or 49.3 ml of sodium bicarbonate solution [7.5%w/v] for each liter of final volume of medium being prepared. Stir until dissolved. While stirring, adjust the pH of the medium to 0.1-0.3 pH units below the desired pH since it may rise during filtration. The use of 1N HCl or 1N NaOH is recommended. Add additional water to bring the solution to final volume. Sterilize immediately by filtration using a membrane with a porosity of 0.22 microns.Aseptically dispense medium into sterile container.
How do I store Product D5523, Dulbecco’s Modified Eagle’s Medium - low glucose, dry powder?  How do I store the reconstituted medium?
Store the dry powdered medium at 2-8 °C under dry conditions and liquid medium at 2-8 °C in the dark.  The nature of supplements added may affect storage conditions and shelf life of the medium. The product label bears the lot-specific expiration date.
What sodium bicarbonate should I use with Product D5523, Dulbecco’s Modified Eagle’s Medium - low glucose?
Sodium Bicarbonate powder (Product No. S5761) or Sodium Bicarbonate Solution, 7.5% (Product No. S8761) is recommended.  For more information, consult the product datasheet at this link:  http://www.sigmaaldrich.com/sigma-aldrich/datasheet/d5523dat.pdf
Do you offer a liquid version of Dulbecco’s Modified Eagle’s Medium - low glucose?
Product No. D6046 is the fully supplemented liquid form of the original Dulbecco's formulation, offered as Product No. D5523, with one key exception.  Pyridoxal, which is an aldehyde, has been replaced with pyridoxine.  This modification has been shown to improve the stability of the medium.  D5546 is the same but does not contain L-glutamine.
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
  What kind of solution is the A5955 - Antibiotic Antimycotic Solution (100×), Stabilized product suspended in?
A5955 - Antibiotic Antimycotic Solution (100×), Stabilized is supsended in a sodium citrate buffer solution. Source: Israel production group
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Protocols & Articles

Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture. The entire contents of each package should be used immediately after opening. Preparing m...

Related Content

Dulbecco's Modified Eagle's Medium - DME, DMEM

Many modifications of Eagle's Medium have been developed since the original formulation appeared in the literature. Among the most widely used of these modifications is Dulbecco's Modified Eagle's me...
Keywords: Cell culture, Substitutions, Vitamins

Peer-Reviewed Papers
15

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