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D5546 Sigma

Dulbecco’s Modified Eagle’s Medium - low glucose

With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym: DME, DMEM

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Properties

Related Categories Cell Biology, Cell Culture, Classic Media and Salts, Classical Media, Culture,
Quality Level   PREMIUM
sterility   sterile-filtered
form   liquid
impurities   endotoxin, tested
suitability   suitable for cell culture
components   HEPES: no
sodium pyruvate: yes
glucose: low
phenol red: yes
L-glutamine: no
NaHCO3: yes
storage temp.   2-8°C

Description

General description

This modification has been shown to improve the stability of the medium.

Reconstitution

Supplement with 0.584 g/L glutamine.

Protocols & Applications

ECACC Handbook-Fundamental Techniques in Cell Culture

Cryostor Cryopreservation Video Protocol

Mycoplasma PCR Detection Kit Video Protocol

Price and Availability


All labs need water

Stericup and Steritop Filter Units
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Related Content

Dulbecco's Modified Eagle's Medium - DME, DMEM

Many modifications of Eagle's Medium have been developed since the original formulation appeared in the literature. Among the most widely used of these modifications is Dulbecco's Modified Eagle's me...
Keywords: Cell culture, Substitutions, Vitamins

Peer-Reviewed Papers
15

References

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