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D5671 Sigma

Dulbecco’s Modified Eagle’s Medium - high glucose

With 4500 mg/L glucose and sodium bicarbonate, without L-glutamine and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture, suitable for hybridoma

Synonym: DME, DMEM

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Properties

Related Categories Cell Biology, Cell Culture, Classic Media and Salts, Classical Media, Core Bioreagents,
Quality Level   PREMIUM
sterility   sterile-filtered
form   liquid
impurities   endotoxin, tested
suitability   suitable for cell culture
  suitable for hybridoma
components   sodium pyruvate: no
glucose: high
L-glutamine: no
NaHCO3: yes
HEPES: no
phenol red: yes
storage temp.   2-8°C

Description

Application

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

Linkage

Need FBS? We can assist with your serum planning. Please visit the Serum Planner for more information.

Reconstitution

Supplement with 0.584 g/L L-glutamine.

General description

This DMEM-Hi glucose medium is a 1x complete medium with no added factors (common modifications) such as L-alanyl-L-glutamine, HEPES, or sodium pyruvate. It differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. This medium requires supplementation with L-glutamine or L-alanyl-L-glutamine.

Protocols & Applications

ECACC Handbook-Fundamental Techniques in Cell Culture

Cryostor Cryopreservation Video Protocol

Mycoplasma PCR Detection Kit Video Protocol

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Discover the Milli-Q® IQ 7000 ultrapure water system
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Fluorescent Live Cell Imaging of Cytoskeleton Structure Proteins using LentiBrite™ Lentiviral Biosensors

Cell analysis of the dynamics of subcellular structures has been revolutionized in the past 15 years by the development and refinement of genetically-encoded fusions between fluorescent proteins (GFP...
Keywords: Apoptosis, Cloning, Fluorescent microscopy, Gene expression, Immunocytochemistry, Immunofluorescence, Microscopy, Rearrangements, Tagged proteins, Transduction, Transfection

Fluorescent Live Cell Imaging of LC3 Mediated Autophagy using LentiBrite™ Lentiviral Biosensors

Autophagy, a degradative pathway that provides recycled nutrients to cells under stress, plays both protective and deleterious roles in many diseases, including cancer, neurodegeneration, and infecti...
Karyn Huryn-Selvar1, Haizhen Liu1, Janet Anderl1, Jun Ma1, Mark Santos1, Matthew Hsu1, and Luke Armstrong1

1 EMD Millipore, 28820 Single Oak Drive, Temecula, CA, USA 92590
Keywords: Autophagy, Cancer, Diseases, Flow cytometry, Fluorescent microscopy, Gene expression, Immunofluorescence, Microscopy, Tagged proteins, Transduction, Transfection

Protocols

Protocol for Transducing Mouse Embryonic Fibroblasts (MEF) using MISSION® ExpressMag™ Super Magnetic Kit®

0.1 Seed 15k MEF per well into 96-well plate. Utilize a final volume of 200 µl of media per well. (Note: for best data interpretation always include an untransduced well that is treated with puromyci...

Related Content

Dulbecco's Modified Eagle's Medium - DME, DMEM

Many modifications of Eagle's Medium have been developed since the original formulation appeared in the literature. Among the most widely used of these modifications is Dulbecco's Modified Eagle's me...
Keywords: Cell culture, Substitutions, Vitamins

Peer-Reviewed Papers
15

References

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