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D5758 Sigma

Diethyl pyrocarbonate

≥97% (NMR)

Synonym: DEP, DEPC, Diethyl dicarbonate, Diethyl oxydiformate, Ethoxyformic acid anhydride

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Properties

Related Categories Biochemicals, Biochemicals and Reagents, Carboxypeptidase P, Core Bioreagents, Enzyme Inhibitors,
InChI Key   FFYPMLJYZAEMQB-UHFFFAOYSA-N
assay   ≥97% (NMR)
refractive index   n20/D 1.398(lit.)
bp   93-94 °C/18 mmHg(lit.)
density   1.101 g/mL at 25 °C(lit.)
storage temp.   2-8°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases.

Diethyl pyrocarbonate has been used in PCR reaction for treating deionized water, which reduces the risk of RNA being degraded by RNases. It is also used for Dot blot hybridization, to dilute total RNA isolated from different micro-organisms.

Biochem/physiol Actions

Inactivates RNase in solution at about 0.1% (v/v), thus protecting RNA against degradation.

DEPC is a potent nuclease inhibitor.0.1% solution of DEPC is used to inactive RNase.
It inhibits the ryanodine binding to ryanodine/Ca2+ receptor channels in skeletal muscle in a dose and time dependent manner and increases the Ca2+ permeability of SR vesicles.

Packaging

5, 25, 50, 100 mL in glass bottle

General description

Diethyl Pyrocarbonate (DEPC) is a chemical use to inactivate RNase enzymes and is sensitive to moisture and pH. It decomposes at 155°C, to ethanol and carbon dioxide in aqueous solution. DEPC is also sensitive to ammonia, which causes decomposition to urethane, a possible carcinogen.

Price and Availability


Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NA 1993 / PGIII
WGK Germany 
3
RTECS 
LQ9350000
Flash Point(F) 
156.2 °F
Flash Point(C) 
69 °C

Frequently Asked Questions

What is the stability of Product D5758, Diethyl pyrocarbonate, at room temperature?
The material is stable at room temperature as long as it is protected from moisture.  We do recommend 2-8 °C for its storage.  To view this information on the Product Information Sheet please click on the following link: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Product_Information_Sheet/d5758pis.Par.0001.File.tmp/d5758pis.pdf
How can I tell if Product D5758, Diethyl pyrocarbonate, has degraded?
DEPC breaks down to ethanol and carbon dioxide.  The carbon dioxide builds up pressure inside the bottle; when opened, it will degas and/or bubble out of the bottle.  To view this information on the Product Information Sheet please click on the following link: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Product_Information_Sheet/d5758pis.Par.0001.File.tmp/d5758pis.pdf
What buffers can I use with Product D5758, Diethyl pyrocarbonate (DEPC)?
DEPC reacts with amines, thiols, and alcohols.  Any buffer that contains an amine, e.g.., Trizma, should not be used.  To view this information on the Product Information Sheet please click on the following link: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Product_Information_Sheet/d5758pis.Par.0001.File.tmp/d5758pis.pdf
What type of container should I use to handle Product D5758, Diethyl pyrocarbonate (DEPC)?
Glassware is preferable since some plastics, e.g.., polycarbonate, are known to react with DEPC.  To view this information on the Product Information Sheet please click on the following link: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Product_Information_Sheet/d5758pis.Par.0001.File.tmp/d5758pis.pdf
Why is stirring or shaking necessary when making RNAse-free water for use with Product D5758, Diethyl pyrocarbonate?
DEPC is not very soluble in water. Stirring and/or shaking is necessary to suspend DEPC in water.
What is the best way to use Product D5758, Diethyl pyrocarbonate, to inactivate RNAse?
Add DEPC directly to the water to yield a 0.1 % solution.  Shake vigorously and allow to set overnight at room temperature with the lid loose or incubated for 2 hours at 37 °C.  The solution is then autoclaved 15 minutes for each liter.  To view this information on the Product Information Sheet please click on the following link: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Product_Information_Sheet/d5758pis.Par.0001.File.tmp/d5758pis.pdf
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles
Peer-Reviewed Papers
15

References

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