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D5796 Sigma

Dulbecco’s Modified Eagle’s Medium - high glucose

With 4500 mg/L glucose, L-glutamine, and sodium bicarbonate, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

Synonym: DME, DMEM

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Description

Application

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

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General description

This DMEM-Hi glucose medium is a 1x complete medium with no added factors (common modifications) such as L-alanyl-L-glutamine, HEPES, or sodium pyruvate. It differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media.

Protocols & Applications

Cryostor Cryopreservation Video Protocol

Price and Availability


CellASIC® ONIX2 System

All labs need water

Stericup and Steritop Filter Units
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

3D Organoid Culture: New In Vitro Models of Development and Disease

Model systems drive biological research by recapitulating body processes and functions from the molecular to whole organism level. The human body is composed of both cellular and non-cellular materia...
Keywords: Adhesion, Cell attachment, Cell culture, Diseases, Growth factors, Infectious Diseases, Processes and Functions, Vitamins

Bile Acids

The liver excretes excess cholesterol in the form of bile acids. Bile acids serve two purposes: to remove unwanted cholesterol from the body and to aid in lipid digestion in the intestine. Bile acids...
BioFiles 2007, 2.7, 17.
Keywords: Absorption, Digestions, Transcription

Protocols

Lentiviral Transduction Protocol

Prepare mammalian cells so that they are growing exponentially and are no more than 70-80% confluent before transduction.
Keywords: Cell culture, Gene expression, Molecular biology, Transduction

Related Content

Dulbecco's Modified Eagle's Medium - DME, DMEM

Many modifications of Eagle's Medium have been developed since the original formulation appeared in the literature. Among the most widely used of these modifications is Dulbecco's Modified Eagle's me...
Keywords: Cell culture, Substitutions, Vitamins

Peer-Reviewed Papers
15

References

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G5792 L-Glutamine, PharmaGrade, Ajinomoto, USP, Manufactured under appropriate GMP controls for pharma or biopharmaceutical production, suitable for cell culture
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