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D5809 Sigma

JumpStart AccuTaq LA DNA Polymerase

Hot-start high fidelity Taq enzyme, 10X buffer included

Synonym: JumpStart® AccuTaq LA DNA Polymerase

Purchase

Properties

Related Categories Core Bioreagents, Hot Start PCR, Life Science Reagents for PCR, Long & Accurate PCR, Molecular Biology,
form   liquid
feature   hotstart
concentration   2.5 unit/μL
color   colorless
  colorless
shipped in   wet ice
storage temp.   −20°C

Description

Application

JumpStart AccuTaq LA DNA Polymerase is a combination of AccuTaq LA DNA Polymerase and a Taq-directed antibody. JumpStart Taq antibody reversibly binds to the AccuTaq LA DNA Polymerase, inactivating it at room temperature. The increased temperature of the first denaturation cycle causes the complex to dissociate, restoring the enzyme activity. This hot start mechanism provides increased specificity and higher target yield in comparison to standard amplification. JumpStart AccuTaq LA DNA Polymerase can generate long products with higher fidelity (up to 6.5× of Taq DNA polymerase).

Features and Benefits

• JumpStart AccuTaq LA DNA polymerase, an antibody inactivated hot start enzyme, is designed to minimize non-specific amplification while increasing target yield & specificity
• Up to 6.5× greater fidelity in comparison to Taq DNA polymerase making it the enzyme of choice for multiplex PCR
• Produce amplicons up to 22 kb with genomic templates and up to 40 kb with less complex templates such as lambda or bacterial genomic DNA
• Reduce set-up time and eliminate concerns associated with manual or wax hot-start methods

Other Notes

View more detailed information on Accutaq products at www.sigma-aldrich.com/hotstart.

Packaging

Supplied with 10× reaction buffer.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

AccuTaq is a trademark of Sigma-Aldrich Co. LLC

Jacobs is a registered trademark of Jacobs

JumpStart is a trademark of Sigma-Aldrich Co. LLC

Unit Definition

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C.

Price and Availability


Ultrapure dNTPs for PCR

Kapa Biosystems

All labs need water
Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Protocols

End Point PCR Protocol for Long and Accurate DNA Amplification

Technology Overview Equipment Reagents Assay Considerations Procedure Troubleshooting Materials References

Related Content

PCR Selection Guide

Sigma-Aldrich offers a wide variety of PCR reagents to meet any experimental needs. Our range of polymerases is customized to meet your End-Point PCR, qPCR, or RT-PCR needs. Our products vary from ro...
Keywords: Polymerase chain reaction, Polymerase chain reaction - quantitative

Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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