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D6165 Sigma

Demecolcine

Hybri-Max, powder, γ-irradiated, hybridoma tested

Synonym: N-Deacetyl-N-methylcolchicine

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Properties

Related Categories Cell Culture, Conditioned Media, Media Supplements and Reagents, Hybridoma Reagents, Reagents and Supplements
grade   Hybri-Max
InChI Key   NNJPGOLRFBJNIW-HNNXBMFYSA-N
sterility   γ-irradiated
form   powder
impurities   endotoxin, tested
suitability   hybridoma tested
storage temp.   2-8°C

Description

Application

Recommended for use in culture medium at a final concentration of 0.4 μg/ml. Incubate cultures for 4-6 hours at 37 °C. Remove medium containing demecolcine, replace with fresh medium.

Reconstitution

Reconstitute 1 mg vial demecolcine with 10 mL of sterile balanced salt solution. After reconstitution, store at 2-8 °C or in frozen aliquots at −20 °C. Repeated freezing and thawing is not recommended.

Biochem/physiol Actions

Often in karyotyping and cell cycle research it is desirable to increase the yield of mitotic cells in a particular phase of the cell cycle. This can be achieved in a variety of ways with the most popular being the use of a cell cycle synchronizing agent such as demecolcine. Demecolcine will arrest cells in metaphase with no remarkable effect on the biochemical events in mitotic cells or in synchronized G1 and S phase cells. White blood cells are often treated with demecolcine to arrest cells in metaphase.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Price and Availability


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Safety & Documentation

Safety Information

Symbol 
GHS06  GHS06
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 2811 6.1 / PGII
WGK Germany 
3
RTECS 
GH0800000

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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Description

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D1925 Demecolcine solution, 10 μg/mL in HBSS, ACF Qualified, BioXtra

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