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  • D6168 - Monoclonal Anti-Dynein (Intermediate Chain) antibody produced in mouse

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D6168 Sigma

Monoclonal Anti-Dynein (Intermediate Chain) antibody produced in mouse

clone 1869A, ascites fluid

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Properties

Related Categories Alphabetical Index, Antibodies, DQ-DZ, Primary Antibodies
biological source   mouse
antibody form   ascites fluid
clone   1869A, monoclonal
contains   15 mM sodium azide
species reactivity   Chlamydomonas, trout (Salmo gairdneri), sea urchin (Tripneustes gratilla), Strongylocentrotus purpuratus
application(s)   western blot: 1:10,000 using Chlamydomonas flagella
  western blot: 1:10000 using Chlamydomonas reinhardtii flagella preparation
isotype   IgG1
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Features and Benefits

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Immunogen

outer arm dynein intermediate chain of Chlamydomonas.

Specificity

Recognizes the intermediate chain (IC69, 69 kDa) of Chlamydomonas dynein, but does not recognize any other protein in the axoneme. Reacts strongly with the 76 kDa intermediate chain outer arm dynein of sea urchin (Tripneustes gratilla, Strongylocentrotus purpuratus) and with a single component (IC2, 73 kDa) of trout (Salmo gairdneri) outer arm dynein. Does not recognize any component in axonemes prepared from scallop gill cilia or sheep sperm, purified 14S and 22S dyneins from cilia of Tetrahymena thermophila, or chick brain extract.

Application

May be used for domain mapping within IC69, electron microscope studies on dynein particles, and for protein-protein association studies on purified outer arm dynein and axonemes from Chlamydomonas flagella.

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Safety & Documentation

Safety Information

WGK Germany 
3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
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