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D6182 Sigma

DOTAP chloride

≥98% (TLC), powder

Synonym: N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium chloride

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Properties

Related Categories Cell Biology, Cell Signaling and Neuroscience, Signal Transduction Research Tools, Transfection Reagents
InChI Key   KSXTUUUQYQYKCR-LQDDAWAPSA-M
assay   ≥98% (TLC)
form   powder
shipped in   dry ice
storage temp.   −20°C

Description

Application

Cationic liposome-forming compound for transfection of DNA, RNA and other negatively charged molecules into eukaryotic cells. Mixing DOTAP with DNA results in spontaneously formed stable complexes that can be directly added to cell culture medium. These complexes fuse with the cell membrane and release DNA into the cytoplasm. The cells are transfected efficiently without cytotoxic effects. Use approximately 5-10 μg DOTAP per μg DNA and approximately 10-20 μg DOTAP per mL cell culture medium. The optimal working concentration and transfection time depends in part on the cell line used, and the type of material (RNA, DNA, protein) loaded.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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