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D6421 Sigma

Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham

With 15 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym: DME/F-12, 1:1 mixture

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Properties

Related Categories Cell Biology, Cell Culture, Classic Media and Salts, Classical Media, Core Bioreagents,
Quality Level   PREMIUM
sterility   sterile-filtered
form   liquid
impurities   endotoxin, tested
suitability   suitable for cell culture
components   L-glutamine: no
NaHCO3: yes
phenol red: yes
HEPES: 15 mM
glucose: 3.15 g/L
sodium pyruvate: 0.055 g/L
storage temp.   2-8°C

Description

Linkage

Need FBS? We can assist with your serum planning. Please visit the Serum Planner for more information.

Reconstitution

Supplement with 0.365 g/L L-glutamine.

Application

DMEM:F12 is a 50:50 mixture of DMEM and Ham’s F12 media that has proven to be useful in a wide range of cell culture applications, especially when supplemented with fetal bovine serum (FBS). Ham′s Nutrient Mixture F12 was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells, and mouse L cells. It is frequently used with dialyzed serum, hormones, selenium, and other supplements for serum-free cultures. It is the medium of choice for supporting the growth of cells of rodent origin, particularly rabbbit and rat, and has been proven to be an excellent cloning medium for myeloma and hybridoma cells.

Suitable for growth of wide range of cell types in low serum conditions.
Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is used in cell culture & proliferation assays. It is also used in bone marrow-derived macrophages culture.

General description

A measured mixture of chemically defined ingredients makes up a basal medium, which is a type of complex media. DMEM is a medium supplemented with serum and sometimes with tryptose phosphate broth (TPB). DMEM/F-12 is a completely defined synthetic medium, which is protein-free.

Protocols & Applications

ECACC Handbook-Fundamental Techniques in Cell Culture

Cryostor Cryopreservation Video Protocol

Mycoplasma PCR Detection Kit Video Protocol

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
Protocols & Articles
Peer-Reviewed Papers
15

References

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