• USA Home
  • D6434 - Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham

EMAIL THIS PAGE TO A FRIEND
D6434 Sigma

Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham

With 15 mM HEPES and sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

Synonym: DME/F-12, 1:1 mixture

Purchase

Properties

Related Categories Cell Biology, Cell Culture, Classic Media and Salts, Classical Media, Culture,
Quality Level   PREMIUM
sterility   sterile-filtered
form   liquid
impurities   endotoxin, tested
suitability   suitable for cell culture
components   sodium pyruvate: 0.055 g/L
glucose: 3.15 g/L
NaHCO3: yes
HEPES: 15 mM
L-glutamine: no
phenol red: no
storage temp.   2-8°C

Description

General description

Pyridoxal, which is an aldehyde, has been replaced with pyridoxine. This modification has been shown to improve the stability of the medium. Suitable for growth of a wide range of cell types in low serum conditions. Absence of phenol red makes this a preferred medium for clonal and stem cell culture.

Reconstitution

Supplement with 0.365 g/L L-glutamine

Protocols & Applications

ECACC Handbook-Fundamental Techniques in Cell Culture

Cryostor Cryopreservation Video Protocol

Mycoplasma PCR Detection Kit Video Protocol

Price and Availability


Stericup and Steritop Filter Units
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles
Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
Related Products

related product

Product #

Description

Add to Cart

08168 Timestrip Plus 8 °C

supplement

Product #

Description

Add to Cart

F2442 Fetal Bovine Serum, USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma
G7513 L-Glutamine solution, 200 mM, solution, sterile-filtered, BioXtra, suitable for cell culture
P3532 Phenol Red, powder, BioReagent, suitable for cell culture

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?