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D6444 Sigma

Anti-DBP5 (N-terminal) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-DDX19B, Anti-DEAD (Asp-Glu-Ala-As) box polypetide 19B, Anti-DEAD5, Anti-DED box protein 19B

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for RNA Regulation and Translation, Antibodies to Ribosomal Proteins and Translation Factors, DA-DD,
species reactivity   human, mouse (predicted), rat (predicted)
application(s)   immunoprecipitation: 5-10 μL using HEK-293T cell lysate
  indirect immunofluorescence: 2.5-5 μg/mL using paraformaldehyde-fixed HEK-293T cells over-expressing human DBP5
  western blot: 1-2 μg/mL using HEK-293T cell lysate
clone   polyclonal
concentration   ~1 mg/mL
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt ~53 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... DDX19B(11269)
mouse ... Ddx19b(234733)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 33-50 of human DBP5, conjugated to KLH. The corresponding sequence differs by three and four amino acids in mouse and rat, respectively.

General description

DBP5 belongs to the family of the DEAD-box helicases that are involved in cellular RNA metabolism from transcription through pre-mRNA splicing, nuclear export, translation initiation to RNA degradation. It localizes within the cytoplasm and at the nuclear rim, where it interacts with components of the nuclear pore complex (NPC). It shuttles between the nucleus and the cytoplasm by using Nup159 as a binding platform.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Anti-DBP5 antibody produced in rabbit is suitable for immunoprecipitation at a working amount of 5-10μL using HEK-293T cell lysate, immunoblotting at a working concentration of 1-2μg/mL using HEK-293T cell lysate and immunofluorescence at a working concentration of 2.5-5μg/mL using paraformaldehyde fixed HEK-293T cells over-expressing human DBP5.

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Biochem/physiol Actions

DBP5 is required for mRNA export from the nucleus in an ATP-dependent manner. It is also involved in translation termination, where it recognizes stop-codon by controlling eRF1-eRF3 interaction.

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
Protocols & Articles

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Peer-Reviewed Papers
15

References

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D6569 Anti-DBP5 (C-terminal) antibody produced in rabbit, ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

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