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D7052 Sigma

Dipeptidyl Peptidase IV from porcine kidney

lyophilized powder, ≥10 units/mg protein (Bradford)

Synonym: Dipeptidyl aminopeptidase IV

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Properties

Related Categories 3.4.x.x Peptidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Dipeptidyl peptidase IV,
form   lyophilized powder
packaging   vial of ≥0.75 unit
solubility   0.1 M Tris-HCl, pH 8.0: soluble1 vial/mL, clear, colorless
storage temp.   −20°C
Gene Information   pig ... DPPIV(397492)

Description

Biochem/physiol Actions

Native DPPIV is a ubiquitous type II transmembrane glycoprotein and a serine protease of the S9 prolyl-oligopeptidase family. In vivo, it is synthesized with a signal peptide, which functions as the membrane anchoring domain. There is an 88% sequence homology between the human and porcine kidney enzymes. Both exist as homodimers with a subunit molecular weight of ~30 kDa. The high mannose 100 kDa DPPIV precursor is processed in the Golgi to yield a 124 kDa heavily N-and O-linked mature glycoprotein. It is then sorted to the apical membrane through the concerted action of both N- and O-linked glycans and its association with lipid microdomains. The porcine enzyme contains 18.3% carbohydrates, which the glycan composition is 0.9% fucose, 3.4% mannose, 5.1% galactose, 8.2% glucosamine, and 0.7% sialic acid. DPPIV is highly expressed on endothelial cells, epithelial cells, and lymphocytes. It is also present in plasma in its soluble form.

The pH optimum for the protease is 7.8-8.0. Dipeptidyl peptidase does not require activators. It is inhibited by diisopropyl fluorophosphate, phenylmethanesulfonyl fluoride, and diethyl p-nitrophenyl phosphate.

Specificity

DPPIV has a post-proline dipeptidyl aminopeptidase activity that hydrolyzes N-terminal dipeptides from the unsubstituted N-terminus of peptides with the sequence of X-Pro-Z and X-Ala-Z. Where X is a nonspecific residue at the N terminus and Z cannot be proline or hydroxyproline.

Unit Definition

One unit will produce 1.0 μmole of p-nitroaniline from Gly-L-Pro p-nitroanilide per min in 100 mM Tris-HCl at pH 8.0 at 37 °C.

Physical form

Lyophilized powder containing Tris buffer salts

Application

Dipeptidyl peptidase IV from porcine kidney has been used in a study to investigate engraftment of donor cells following hepatocyte transplantation. Dipeptidyl peptidase IV from porcine kidney has also been used in a study to investigate the metabolism of glucagon-like peptide-2.

The enzyme from sigma has been used in the determination of DPP-IV inhibitory activity of peptides in porcine skin gelatin hydrolysates.

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Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Plasma Derived Proteins and Enzymes

Despite their complexity, blood and plasma are abundant biological resources for the discovery of drug targets and biomarkers for human disease. It is estimated that plasma may contain as many as 40,...
BioFiles 2006, 1.5, 2.
Keywords: Applications, Gas chromatography, Type

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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inhibitor

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K4264 KR-62436 hydrate, ≥95%, powder
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substrate

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G2901 Gly-Pro p-nitroanilide p-toluenesulfonate salt, ~99% (TLC)
G2761 Gly-Pro-7-amido-4-methylcoumarin hydrobromide, dipeptidylpeptidase IV substrate
G9262 Gly-Pro 4-methoxy-β-naphthylamide hydrochloride, powder
G9137 Gly-Pro 4-methoxy-β-naphthylamide, dipeptidylpeptidase IV substrate
G0513 Gly-Pro p-nitroanilide hydrochloride, ≥99% (HPLC)

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