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D7058 Sigma

DirectLoad Wide Range DNA Marker

ready-to-use marker for DNA electrophoresis

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Description

Components

Sigma’s DirectLoad Wide Range DNA Marker is provided in a gel-loading solution of 2.5% Ficoll (Type 400), 0.0125% bromophenol blue, and 0.00625% xylene cyanol.

Application

Suitable for size determination of PCR-generated DNA fragments with either agarose or polyacrylamide gels.

Features and Benefits

• Ready-to-load
• Easy-to-use
• Popular band sizes

General description

Sigma’s DirectLoad Wide Range DNA Marker contains 16 fragments from 50 – 10,000 bp, with bands regularly spaced. Ten ul of the ladder can be loaded directly in a single lane on an agarose or polyacrylamide gel.

Other Notes

Contains 16 fragments, 50–10,000 bp which produce logically spaced bands on an agarose gel.

For optimal resolution, the recommended agarose gel concentration is 0.75 – 2.0%.

Legal Information

DirectLoad is a trademark of Sigma-Aldrich Co. LLC

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Articles

Introduction to Markers and Ladders

Sigma-Aldrich offers a variety of markers that aid size determination of samples separated by agarose and/or polyacrylamide gel electrophoresis. These products include markers for DNA, PCR fragments ...
Keywords: Digestions, Electrophoresis, Gel electrophoresis, PAGE, Polymerase chain reaction

Introduction to Nucleic Acid Electrophoresis

Introduction Agarose gel electrophoresis for DNA Agarose gel electrophoresis for RNA Polyacrylamide gel electrophoresis for DNA Reference
Keywords: AGE, Buffers, Degradations, Electrophoresis, Environmental, Gel electrophoresis, PAGE, Purification, Separation, Size-exclusion chromatography

Markers and Ladders Selection Guide

DNA and RNA size markers contain a mixture of DNA (or RNA) fragments of known length, making them suitable for estimating the fragment length of concurrently run samples. They stain well with ethidiu...
Keywords: AGE, Electrophoresis, Gel electrophoresis, PAGE, Polymerase chain reaction

Protocols

End Point PCR Protocol for Long and Accurate DNA Amplification

Technology Overview Equipment Reagents Assay Considerations Procedure Troubleshooting Materials References

Peer-Reviewed Papers
15

References

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D3812 DirectLoad 50 bp DNA Step Ladder, ready-to-use marker for DNA electrophoresis
D3937 DirectLoad 1 kb DNA Ladder, ready-to-use marker for DNA electrophoresis
D0428 1 kb DNA Ladder, for DNA electrophoresis

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