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D7291 Sigma

Deoxyribonuclease I RNase-free solution from bovine pancreas

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Properties

Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Enzyme Class Index,
grade   for molecular biology
form   buffered aqueous glycerol solution
mol wt   mol wt 29.1 kDa
foreign activity   RNase, none detected
storage temp.   −20°C
Gene Information   cow ... DNASE1(282217)

Description

Components

DNase I is provided in a solution of 50% (v/v) glycerol, 20 mM sodium acetate (pH 6.5), 5 mM CaCl2, and 0.1 mM PMSF.

General description

Digests single- and double-stranded DNA to a mixture of mono- and oligonucleotides carrying 5′ phosphates and 3′ OH termini. This catalytic activity is divalent ion-dependent. In the presence of Mg2+, DNase I hydrolyzes each strand of double-stranded DNA randomly and independently. In the presence of Mn2+, both strands can be cleaved.

Other Notes

RNA treated with this DNase I should not be used to generate a cDNA library or used for RT-PCR. For these sensitive applications, it is recommended to use DNase I, Amplification Grade , Catalog Number AMP-D1.

Unit Definition

One unit will cause a ΔA260 of 0.001 per min per mL reaction mixture using calf thymus DNA as substrate.

Application

Used in molecular biology applications for removing DNA during RNA purification, for preparing DNA for nick translation, and for DNA-protein interaction analysis by footprinting methods.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Protocols

Deoxyribonuclease I

Catalog Number: D7291 Storage Temperature –20 °C CAS RN: 9003-98-9 EC 3.1.21.1 Synonym: DNase I
Keywords: Amplification, Catalysis, Degradations, Digestions, Electrophoresis, Gel electrophoresis, PAGE, Polymerase chain reaction, Purification, RNA purification

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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