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D7304 Sigma

3,3′-Diaminobenzidine (DAB) Liquid Substrate System tetrahydrochloride

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Description

Caution

Stable at least one year at 2-8 °C.

Quantity

Each DAB Liquid Substrate System will provide 250 mL of DAB substrate solution.

Application

DAB Liquid Substrate System is a precipitating substrate system for peroxidase. It produces a brown precipitate for use in immunohistological and immunoblotting staining procedures. Not recommended for ELISA (microwell) procedures. DAB has been used as a substrate in the immunoblot assay carried out to identify the changes in heat shock proteins (Hsps) expressed in the liver. It has been used as a substrate for visualization of sections after immunostaining.

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10X DAB Liquid Chromogen 25 mL SDS    
ready-to-use buffer/peroxide solution 250 mL SDS    
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 3316 9
Protocols & Articles

Articles

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrat...
BioFiles 2008, 3.4, 6.
Keywords: Applications, Enzyme-linked immunosorbent assay, Formulations, Immunohistochemistry, Western blot

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
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