|Related Categories||Apoptosis and Cell Cycle, Bioactive Small Molecules, Cell Biology, Cell Signaling and Neuroscience, Cytoskeleton and Extracellular Matrix,|
|solubility||chloroform: soluble10 mg/mL|
Depolymerizes microtubules; blocks mitosis at metaphase.
Often in karyotyping and cell cycle research it is desirable to increase the yield of mitotic cells in a particular phase of the cell cycle. This can be achieved in a variety of ways with the most popular being the use of a cell cycle synchronizing agent such as demecolcine. Demecolcine will arrest cells in metaphase with no remarkable effect on the biochemical events in mitotic cells or in synchronized G1 and S phase cells. White blood cells are often treated with demecolcine to arrest cells in metaphase.
Bottomless glass bottle. Contents are inside inserted fused cone.
Certificate of Analysis
Certificate of Origin
|Precautionary statements||P264-P301 + P310|
|Personal Protective Equipment||Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges|
|Hazard Codes (Europe)||T|
|Risk Statements (Europe)||25|
|Safety Statements (Europe)||22-24/25-45|
|RIDADR||UN 2811 6.1 / PGII|
Mars, A Drosophila Protein Related To Vertebrate HURP, Is Required For The Attachment Of Centrosomes To The Mitotic Spindle During Syncytial Nuclear Divisions. Zhang, G., et al. J. Cell Biol. 122, 535-45, (2009)
RNAi mediated acute depletion of retinoblastoma protein (pRb) promotes aneuploidy in human primary cells via micronuclei formation. Amato A, Lentini L, Schillaci T, et al. BMC Cell Biol. 10, 79, (2009)
The SUMO protease SENP1 is required for cohesion maintenance and mitotic arrest following spindle poison treatment. Era S, Abe T, Arakawa H, et al. Biochem. Biophys. Res. Commun. 426(3), 310-6, (2012)
DNA-unstable decaploid mouse H1 (ES) cells established from DNA-stable pentaploid H1 (ES) cells polyploidized using demecolcine. Fujikawa-Yamamoto K, Miyagoshi M, Luo X, et al. Cell Prolif. 44(2), 111-9, (2011)
Megakaryocyte lineage-specific class VI β-tubulin suppresses microtubule dynamics, fragments microtubules, and blocks cell division. Yang H, Ganguly A, Yin S, et al. Cytoskeleton (Hoboken.) 68(3), 175-87, (2011)
Demecolcine- and nocodazole-induced enucleation in mouse and goat oocytes for the preparation of recipient cytoplasts in somatic cell nuclear transfer procedures. Costa-Borges N, Paramio MT, Santaló J, et al. Theriogenology 75(3), 527-41, (2011)
Short-term treatment with 6-DMAP and demecolcine improves developmental competence of electrically or Thi/DTT-activated porcine parthenogenetic embryos. Park SJ, Koo OJ, Kwon DK, et al. Zygote 19, 1-8, (2011)
MPF governs the assembly and contraction of actomyosin rings by activating RhoA and MAPK during chemical-induced cytokinesis of goat oocytes. Wu YG, Zhou P, Lan GC, et al. PLoS ONE 5(9), e12706, (2010)
Assessment of the mutagenic activity of extracts of brazilian propolis in topical pharmaceutical formulations on Mammalian cells in vitro and in vivo. Juliana Marques Senedese et al Evid. Based. Complement. Alternat. Med. 2011, DOI: 10.1093/ecam/nen049, (2011)
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