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D7440 Sigma

JumpStart Taq ReadyMix for Quantitative PCR

For probe-based real-time PCR



Related Categories Amplification, Cell Biology, Core Bioreagents, Functional Profiling of Stem Cells, General Stem Cell Biology,
form   liquid
feature   hotstart
concentration   1.5 units/reaction (50 μL reaction volume)
color   colorless
Featured Industry   Agriculture
compatibility   for use with ABI 5700
  for use with ABI 7000
  for use with ABI 7300
  for use with ABI 7500 Fast
  for use with ABI 7500
  for use with ABI 7700
  for use with ABI 7900 Fast
  for use with ABI 7900 HT
  for use with ABI 7900
  for use with ABI StepOne
  for use with ABI StepOnePlus
  for use with ABI ViiA 7
  for use with Bio-Rad CFX384
  for use with Bio-Rad CFX96
  for use with Bio-Rad MJ Chromo4
  for use with Bio-Rad MJ Opticon 2
  for use with Bio-Rad MJ Opticon Cepheid SmartCycler
  for use with Bio-Rad MJ Opticon
  for use with Bio-Rad MiniOpticon
  for use with Eppendorf® Mastercycler ep realplex2 s
  for use with Eppendorf® Mastercycler ep realplex
  for use with Illumina Eco qPCR
  for use with Qiagen Corbett Rotor-Gene 3000
  for use with Qiagen Corbett Rotor-Gene 6000
  for use with Qiagen Corbett Rotor-Gene Q
  for use with Roche LightCycler 480
  for use with Strategene Mx3000P
  for use with Strategene Mx3005P
  for use with Strategene Mx4000
shipped in   wet ice
storage temp.   −20°C


Features and Benefits

• Ultimate Convenience. ReadyMixes contain all components necessary for QPCR, simply add fluorescent detection chemistry, primers, and template
• Greater Specificity & Increased Target Yield. JumpStart Taq prevents non-specific amplification and increased target yield
• Maximum Flexibility. Suitable for use with a variety of detection chemistries including molecular probes and double-stranded binding dyes such as SYBR Green I

Other Notes

JumpStart Taq ReadyMix for Quantitative PCR combines the advantages of a hot start enzyme with a ready-to-use mix for high throughput, quantitative PCR (qPCR). It is formulated without a detection chemistry making it suitable for use with a variety of formats including dual-labeled probes, molecular beacons, or double stranded binding dyes such as SYBR Green I.


Sigma′s Reference Dye for Quantitative PCR is included separately with this ReadyMix for normalization of the reaction data. The dye has a maximum excitation of 586 nm, and a maximum emission of 605 nm. The instrument settings for ROX reference dye are satisfactory for the measurement of the Reference Dye for Quantitative PCR. A tube of 25 mM MgCl2 is also provided for easy optimization of the qPCR reaction.

Default reaction volume is 50 μL

20RXN is packaged as 1 X 500 μL
100RXN is packaged as 1 X 2.5 mL
400RXN is packaged as 1 X 10 mL


The ReadyMix contains JumpStart Taq DNA Polymerase, 99% pure deoxynucleotides, and buffer in an optimized 2× concentrate. To prepare a reaction, 25 μL of the ReadyMix is added to primers, template, detection chemistry, and water for a total reaction volume of 50 μL. Set up can be performed at room temperature since the JumpStart Taq antibody renders the Taq DNA polymerase inactive. During the first denaturation cycle, the antibody dissociates from the enzyme and full activity is restored. No special preparations or protocol changes are required.

Legal Information

A license to perform the patented 5′ Nuclease Process for research is obtained by the purchase of (i) both Authorized 5′ Nuclease Core Kit and Licensed Probe, (ii) a Licensed 5′ Nuclease Kit, or (iii) license rights from Applied Biosystems.

This product is an Authorized 5′ Nuclease Core Kit. Use of this product is covered by one or more of the following claims outside the US corresponding to US Patent No. 5,210,015 and 5,487,972. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. Separate purchase of a Licensed Probe would convey rights under US Patents and corresponding patent claims outside the US: 5,538,848, 5,723,591, 5,876,930, 6,030,787, 6,258,569, 5,804,375 (claims 1-12 only), and and claims outside the United States corresponding to US Patent No. 6,214,979. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained from the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Eppendorf is a registered trademark of Eppendorf AG

JumpStart is a trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Unit Definition

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C.


JumpStart Taq ReadyMix for Quantitative PCR has been used for qPCR amplifications using probes (during assay of HIV-1 RNA, DNA and inflammatory mRNAs). It has been used for Real-time PCR analysis of total extracted RNA in a study to monitor the activity of licorice in Treg cell differentiation and function.

Price and Availability

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Your Key to Success

Standard component


Product #

Add to Cart

JumpStart Taq ReadyMix, Complete optimized reagent for hot-start PCR at 2X concentration 2 X SDS P2893
25 mM MgCl2 1.5 μL SDS M8787
Reference Dye for Quantitative PCR, 100 ×, solution 100 X SDS R4526
Safety & Documentation
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