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D7440 Sigma

JumpStart Taq ReadyMix for Quantitative PCR

For probe-based real-time PCR

Purchase

Properties

Related Categories Amplification, Cell Biology, Core Bioreagents, Functional Profiling of Stem Cells, General Stem Cell Biology,
form   liquid
feature   hotstart
concentration   1.5 units/reaction (50 μL reaction volume)
color   colorless
Featured Industry   Agriculture
compatibility   for use with ABI 5700
  for use with ABI 7000
  for use with ABI 7300
  for use with ABI 7500 Fast
  for use with ABI 7500
  for use with ABI 7700
  for use with ABI 7900 Fast
  for use with ABI 7900 HT
  for use with ABI 7900
  for use with ABI StepOne
  for use with ABI StepOnePlus
  for use with ABI ViiA 7
  for use with Bio-Rad CFX384
  for use with Bio-Rad CFX96
  for use with Bio-Rad MJ Chromo4
  for use with Bio-Rad MJ Opticon 2
  for use with Bio-Rad MJ Opticon Cepheid SmartCycler
  for use with Bio-Rad MJ Opticon
  for use with Bio-Rad MiniOpticon
  for use with Eppendorf® Mastercycler ep realplex2 s
  for use with Eppendorf® Mastercycler ep realplex
  for use with Illumina Eco qPCR
  for use with Qiagen Corbett Rotor-Gene 3000
  for use with Qiagen Corbett Rotor-Gene 6000
  for use with Qiagen Corbett Rotor-Gene Q
  for use with Roche LightCycler 480
  for use with Strategene Mx3000P
  for use with Strategene Mx3005P
  for use with Strategene Mx4000
shipped in   wet ice
storage temp.   −20°C

Description

Features and Benefits

• Ultimate Convenience. ReadyMixes contain all components necessary for QPCR, simply add fluorescent detection chemistry, primers, and template
• Greater Specificity & Increased Target Yield. JumpStart Taq prevents non-specific amplification and increased target yield
• Maximum Flexibility. Suitable for use with a variety of detection chemistries including molecular probes and double-stranded binding dyes such as SYBR Green I

Other Notes

JumpStart Taq ReadyMix for Quantitative PCR combines the advantages of a hot start enzyme with a ready-to-use mix for high throughput, quantitative PCR (qPCR). It is formulated without a detection chemistry making it suitable for use with a variety of formats including dual-labeled probes, molecular beacons, or double stranded binding dyes such as SYBR Green I.

Packaging

Sigma′s Reference Dye for Quantitative PCR is included separately with this ReadyMix for normalization of the reaction data. The dye has a maximum excitation of 586 nm, and a maximum emission of 605 nm. The instrument settings for ROX reference dye are satisfactory for the measurement of the Reference Dye for Quantitative PCR. A tube of 25 mM MgCl2 is also provided for easy optimization of the qPCR reaction.

Default reaction volume is 50 μL

20RXN is packaged as 1 X 500 μL
100RXN is packaged as 1 X 2.5 mL
400RXN is packaged as 1 X 10 mL

Principle

The ReadyMix contains JumpStart Taq DNA Polymerase, 99% pure deoxynucleotides, and buffer in an optimized 2× concentrate. To prepare a reaction, 25 μL of the ReadyMix is added to primers, template, detection chemistry, and water for a total reaction volume of 50 μL. Set up can be performed at room temperature since the JumpStart Taq antibody renders the Taq DNA polymerase inactive. During the first denaturation cycle, the antibody dissociates from the enzyme and full activity is restored. No special preparations or protocol changes are required.

Legal Information

A license to perform the patented 5′ Nuclease Process for research is obtained by the purchase of (i) both Authorized 5′ Nuclease Core Kit and Licensed Probe, (ii) a Licensed 5′ Nuclease Kit, or (iii) license rights from Applied Biosystems.

This product is an Authorized 5′ Nuclease Core Kit. Use of this product is covered by one or more of the following claims outside the US corresponding to US Patent No. 5,210,015 and 5,487,972. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. Separate purchase of a Licensed Probe would convey rights under US Patents and corresponding patent claims outside the US: 5,538,848, 5,723,591, 5,876,930, 6,030,787, 6,258,569, 5,804,375 (claims 1-12 only), and and claims outside the United States corresponding to US Patent No. 6,214,979. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained from the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Eppendorf is a registered trademark of Eppendorf AG

JumpStart is a trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Unit Definition

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C.

Application

JumpStart Taq ReadyMix for Quantitative PCR has been used for qPCR amplifications using probes (during assay of HIV-1 RNA, DNA and inflammatory mRNAs). It has been used for Real-time PCR analysis of total extracted RNA in a study to monitor the activity of licorice in Treg cell differentiation and function.

Price and Availability


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Standard component

Description

Product #

Add to Cart

JumpStart Taq ReadyMix, Complete optimized reagent for hot-start PCR at 2X concentration 2 X SDS P2893
25 mM MgCl2 1.5 μL SDS M8787
Reference Dye for Quantitative PCR, 100 ×, solution 100 X SDS R4526
Safety & Documentation
Protocols & Articles

Articles

Improving Real-Time PCR Success

To meet increasing food supply demands of the global population, growers require seeds that can withstand the regional challenges found in their fields. In order to develop crop varieties that are mo...
Keywords: Agriculture, Amplification, Environmental, Polymerase chain reaction

Metagenomics and Quantitative PCR

The highly variable regions within the 16S ribosomal RNA (rRNA) gene sequences are being exploited with PCR and metagenomic sequencing to characterize the microbial strains within the gastrointestina...
Chloe McClanahan
Biofiles Edition 6.2
Keywords: Gastrointestinal, Nucleic acid hybridization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Probiotics, Sequences, Sequencing, Usage

Polymerase Chain Reaction - PCR Technologies Guide

The polymerase chain reaction (PCR)1,2,3 has become one of the most widely used techniques in molecular biology. It is used in applications from basic research to high-throughput screening. While it ...
Keywords: Amplification, Cloning, Degradations, Detection methods, Electrophoresis, Evaporation, Gas chromatography, Gel electrophoresis, Indicators, Melting, Molecular biology, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Sequencing, Size-exclusion chromatography, Transcription

Protocols

Multiplex qPCR with JumpStart™ Taq ReadyMix™ for Quantitative PCR

Multiplex qPCR is a technique that simultaneously amplifies two or more target sequences in a single reaction. Analysis of multiple targets per sample allows for conservation of limited starting mate...
Keywords: AGE, Amplification, Electrophoresis, Gas chromatography, Gel electrophoresis, Gene expression, Melting, Poisons, Polymerase chain reaction, Polymerase chain reaction - quantitative

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Sigma-Aldrich products are aligned to the discovery, development and production phases of the plant breeding workflow, allowing you to develop and produce new crop varieties faster. With materials ma...

Automated Protocols for Life Science Research

Scientists at Sigma-Aldrich® have validated all of the automated methods available below. Automation protocols are also provided as instruction for each of the methods. These protocols supply informa...
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PCR Selection Guide

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Peer-Reviewed Papers
15

References

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